5-Hydroxytryptamine1A receptor binding activity of bisbenzyltetrahydroisoquinoline alkaloids from Popowia odoardi

Chung, L.Y.; Soo, W. K.; Mustafa, M. R.; Goh, S. H.; Imiyabir, Z. (2009) 5-Hydroxytryptamine1A receptor binding activity of bisbenzyltetrahydroisoquinoline alkaloids from Popowia odoardi. Pharmaceutical Biology, 47 (12). pp. 1130-1136. ISSN 1388-0209

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    Abstract

    A 96-well filtration-based 5-HT1A receptor-radioligand binding assay was optimized and adapted to carry out a bioassay-guided fractionation of the methanol extract of the bark of Popowia odoardi Diels(Annonaceae). This purification led to the isolation of two compounds identified as O-methyldauricine (1)and popisidine (2), which bind competitively to 5-HT 1A receptors at high concentrations with K ivalues of 4.5 and 5.8 μM, with [ 3 H]8-OH-DPAT as the radioligand. These results suggest that both of these bisbenzyltetrahydroisoquinoline alkaloids influence the activity of 5-HT 1A receptors through competitive binding as agonists or antagonists and may possess anxiolytic and anti-depressive effects.

    Item Type: Article
    Creators:
    1. Chung, L.Y.
    2. Soo, W. K.
    3. Mustafa, M. R.
    4. Goh, S. H.
    5. Imiyabir, Z.
    Journal or Publication Title: Pharmaceutical Biology
    Additional Information: Address for Correspondence: L. Y. Chung, Department of Pharmacy, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia. Tel.: +603 79674971; Fax: +603 79674964; E-mail: chungly@um.edu.my
    Uncontrolled Keywords: Alkaloid; Annonaceae; 5-hydroxytryptamine Receptor; O-methyldauricine; Popowia Odoardi; Popisidine
    Subjects: Q Science > QD Chemistry
    R Medicine
    Divisions: Faculty of Medicine
    Depositing User: Ms Haslinda Lahuddin
    Date Deposited: 17 Jan 2012 12:29
    Last Modified: 17 Jan 2012 12:29
    URI: http://eprints.um.edu.my/id/eprint/2454

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