Inadvertent expression of dengue 2 virus NS3-EGFP fusion protein in Escherichia coli using the pEGFP-N1 mammalian expression vector

Shafee, Norazizah and AbuBakar, Sazaly (1999) Inadvertent expression of dengue 2 virus NS3-EGFP fusion protein in Escherichia coli using the pEGFP-N1 mammalian expression vector. Journal of the University of Malaya Medical Centre (JUMMEC), 4 (1). pp. 43-46. ISSN 1823-7339,

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Official URL: https://jummec.um.edu.my/article/view/4431

Abstract

Dengue 2 New Guinea C (NGC) virus NS3 protein, a potentially important virulence factor was cloned to the N-terminus of the Aequoreo t.rictorio enhanced green fluorescent protein (EGFP) using the pEGFP-N1 manimaiian expression vector. During amplification of the recombinant plasmid in E. coli, transformants expressing the EGFI' were detected in vivo when viewed using fluorescence microscopy. This inadvertent expression of the recombinant fusion protein was confirmed further by detection of the T7-Tag peptide cloned to the amino terminal of the fusion protein using T7-Tag specific monoclonal antibody. These findings represent perhaps the first reported expression of the T7-Tag-N53-EGFP fusion protein using the pEGFP-N1 mammalian expression vector in E. coli.

Item Type: Article
Funders: UNSPECIFIED
Uncontrolled Keywords: Dengue; NS3; pEGFP-N1; fusion protein
Subjects: R Medicine
Divisions: Faculty of Medicine
Depositing User: Mr Jasny Razali
Date Deposited: 22 Mar 2021 05:14
Last Modified: 22 Mar 2021 05:14
URI: http://eprints.um.edu.my/id/eprint/24513

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