Muscarinic receptor activity of some Malaysian plant species

Chung, L.Y.; Yap, K.F.; Mustafa, M.R.; Goh, S.H.; Imiyabir, Z. (2005) Muscarinic receptor activity of some Malaysian plant species. Pharmaceutical Biology, 43 (8). pp. 672-682. ISSN 1388-0209

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    Muscarinic receptor binding activity was tested on 224 plant extracts obtained from more than 50 plant families found in Malaysia. The plant extracts were evaluated by a 96-well microplate filtration–based radioligand competitive assay, centered on the ability of the plant extracts to competitively displace the radioligand, [3 H]N-methylscopolamine, from binding to the muscarinic membrane receptors. The screening assay was initially carried out at 50 mg=assay point, and those showing inhibition at and above 61% were retested at 10 mg=assay point. The extracts of Ficus septica Burm. f. (Moraceae) [65.85 � 3.75% inhibition; mean (n ¼ 3) � SD], Polyalthia microtus Miq. (Annonaceae) (32.63 � 1.38% inhibition), and Popowia odoardoi Diels (Annonaceae) (35.79 � 7.11% inhibition) at 10 mg=assay point exhibited muscarinic properties, which are worthy of further investigation.

    Item Type: Article
    1. Chung, L.Y.
    2. Yap, K.F.
    3. Mustafa, M.R.
    4. Goh, S.H.
    5. Imiyabir, Z.
    Journal or Publication Title: Pharmaceutical Biology
    Additional Information: Address correspondence to: L.Y. Chung, Department of Pharmacy, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia. E-mail:
    Uncontrolled Keywords: Ficus Septica, Malaysian Plants, Muscarinic Receptor, Polyalthia Microtus, Popowia Odoardoi, Radioligand Binding Assay
    Subjects: R Medicine
    R Medicine > R Medicine (General)
    Divisions: Faculty of Medicine
    Depositing User: Ms Haslinda Lahuddin
    Date Deposited: 17 Jan 2012 11:20
    Last Modified: 20 Oct 2014 13:36

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