Biological activities of dengue virus on murine macrophages

Rajamani, Leela and Aziz, Nadia and Sekaran, Shamala Devi (1997) Biological activities of dengue virus on murine macrophages. Journal of the University of Malaya Medical Centre (JUMMEC), 2 (1). pp. 11-17. ISSN 1823-7339

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Official URL: https://jummec.um.edu.my/article/view/4398

Abstract

The biological activities of dengue 2 virus on its target cells, namely macrophages and neutrophils were investigated. Preliminary results showed that polyethylene glycol precipitated dengue 2 virus acted as a chemoattractant for peritoneal cells of Balbc/I mice and for established rnonoctyesfmacrophage cell lines (WEHI 265.1). This in nine response was also seen in vitro using Boyden Chemotaxis chambers and was found to be inhibited by specific viral antibodies. The ability of the virus to activate these cells was then evaluated. Treatment of the peritoneal cells with virus induced a respiratory burst with the production of picomoles of superoxides. In addition, the virus induced production of reactive nitrogen intermediates (1-2 uM) and also resulted in exocytosis of Iysozyme. Adhesiveness was also observed and appeared to increase with higher concentrations of the virus. The above results demonstrate that increased adhesiveness and activation are induced following treatment of peritoneal cells and the cell line WEHI 265.1 with dengue virus.

Item Type: Article
Uncontrolled Keywords: Macrophages; macrophages activation; dengue virus; adhesion
Subjects: R Medicine
Divisions: Faculty of Medicine
Depositing User: Mr Jasny Razali
Date Deposited: 01 Jun 2020 00:47
Last Modified: 01 Jun 2020 00:47
URI: http://eprints.um.edu.my/id/eprint/24376

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