Immunoexpression of pRb and EGFR using tissue microarray (TMA) technique - a preliminary study

Kipli, N.P.; Cheong, C.S.; Zain, R.B.; Hamid, S.; Lim, K.P.; Abraham, T.; Ismail, M.S.; Hussaini, H.M. (2006) Immunoexpression of pRb and EGFR using tissue microarray (TMA) technique - a preliminary study. Journal Of Oral Pathology & Medicine, 35 (7). p. 450. ISSN 0904-2512

[img]
Preview
PDF [error in script]
Download (83Kb) | Preview

    Abstract

    Purpose: To assess the expression of pRb and EGFR in oral epithelial lesions using TMA technique. Methods: Paraffin embedded specimens were selected from the archives of the Diagnostic Laboratories at the Faculty of Dentistry, University of Malaya and Universiti Kebangsaan Malaysia. Selected epithelial areas were cored and developed into tissue microarray blocks. A total of 32 samples for pRb and 50 samples for EGFR were analysed using immunohistochemical techniques. Results: Abnormal pRb staining (1? or less) was seen in 80% (8/10) OSCC, 38.5% (5/13) dysplastic and 22.2% (2/9) normal/hyperplastic epithelium. Positive EGFR staining was observed in 25/35 (71.4%) OSCC cases with 11 cases (31.4%) showing staining of 2? or more. Out of 5 epithelial dysplasia and 10 normal/hyperplastic epithelium, positive EGFR staining was noted in 3 (60%) and 8 (80%) cases respectively. Interestingly, only 1(20%) epithelial dysplasia and 1(10%) normal/hyperplastic epithelium showed staining of 2? or more. Conclusion: High frequency of abnormal expression of pRb is observed in OSCC cases as compared to dysplastic and normal/hyperplastic epithelium. EGFR is expressed in OSCC as well as epithelial dysplasia and normal/hyperplastic epithelium. However, higher EGFR positivity is more common in OSCC cases. This study supports the feasibility and ease of using TMA technique for immunohistochemical studies.

    Item Type: Article
    Creators:
    1. Kipli, N.P.
    2. Cheong, C.S.
    3. Zain, R.B.(Department of Oral Pathology and Oral Medicine and Periodontology, Faculty of Dentistry, University of Malaya)
    4. Hamid, S.
    5. Lim, K.P.
    6. Abraham, T.
    7. Ismail, M.S.
    8. Hussaini, H.M.
    Journal or Publication Title: Journal Of Oral Pathology & Medicine
    Additional Information: Prof. Dr. Rosnah Mohd Zain Department of Oral Pathology and Oral Medicine and Periodontology, Faculty of Dentistry, University of Malaya
    Uncontrolled Keywords: Oral Squamous Cell Carcinoma, Lichenoid Lesions, Lichen Planus, Oral Cancer, Oral Tumours, Pemphigus, Traumatic Eosinophilic Granuloma, Aphthous Ulcers, Oral Mucosal Lesions, Betel Chewers Mucosa, Betel Quid Related Lesions, Betel Quid, Areca Quid, Tobacco Quid, Oral Cancer Screening, Training and Calibration, Early Detection, Oral Cancer Awareness, Biobanking, Tissue Bank, Databank, Oral Cancer, Tissue Bank, Research Credibility, Research Ethics.
    Subjects: R Medicine
    R Medicine > RK Dentistry
    R Medicine > RK Dentistry > Oral surger
    Divisions: Faculty of Dentistry > Dept of Oral Pathology & Oral Medicine & Periodontology
    Depositing User: Prof. Dr. Rosnah Mohd Zain
    Date Deposited: 12 Jan 2012 09:23
    Last Modified: 11 Dec 2013 15:29
    URI: http://eprints.um.edu.my/id/eprint/2414

    Actions (For repository staff only: Login required)

    View Item

    Document Downloads

    More statistics for this item...