In vitro antiviral activity of fisetin, rutin and naringenin against dengue virus type-2

Zandi, Keivan and Teoh, Boon Teong and Sam, Sing Sin and Wong, Pooi Fong and Mustafa, Mohd Rais and AbuBakar, Sazaly (2011) In vitro antiviral activity of fisetin, rutin and naringenin against dengue virus type-2. Journal of Medicinal Plants Research, 5 (23). pp. 5534-5539. ISSN 1996-0875

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Abstract

In vitro antiviral activities of three flavonoids; fisetin, naringenin and rutin against DENV-2 (NGC strain) were evaluated. Inhibitory effects of each compound at the different stages of DENV-2 infection were examined using foci forming unit reduction assay (FFURA) and quantitative real-time polymerase chain amplification (qRT-PCR). Fisetin, rutin and naringenin showed cytotoxic effects against Vero cells with 50% cytotoxicity (CC50) values of 247, >1000, and 87 µg/mL, respectively. Fisetin when added to Vero cells after virus adsorption inhibited DENV replication with a half maximal inhibition concentration (IC50) value of 55 µg/mL and selectivity index (SI) of 4.49. The IC50 value of fisetin was 43.12 µg/mL with SI=5.72 when Vero cells were treated for 5 h before virus infection and continuously up to 4 days postinfection. There was no direct virucidal activity or prophylactic activity of fisetin against DENV-2. Rutin and naringenin did not inhibit DENV-2 replication in Vero cells. Naringenin however, exhibited direct virucidal activity against DENV-2 with IC50 = 52.64 µg/mL but the SI was <1. The present study suggests that among the flavonoids examined, only fisetin showed significant in vitro anti dengue virus replication activity.

Item Type: Article
Additional Information: Tropical Infectious Diseases Research and Education Center (TIDREC), Department of Medical Microbiology, Faculty of Medicine, University Malaya, Kuala Lumpur, Malaysia
Uncontrolled Keywords: Infectious Disease; Virology; Dengue; Antivirals; Flavonoid.
Subjects: R Medicine
S Agriculture > SB Plant culture
Divisions: Faculty of Medicine
Depositing User: Ms Haslinda Lahuddin
Date Deposited: 11 Jan 2012 07:10
Last Modified: 18 Dec 2019 06:32
URI: http://eprints.um.edu.my/id/eprint/2412

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