Standardized bioassays: An improved method for studying Fusarium oxysporum f. sp. cubense race 4 (FocR4) pathogen stress response in musa acuminata cv. ‘Berangan’

Mohd-Yusuf, Yusmin and Khalid, Norzulaani and Al-Obaidi, Jameel R. and Baharum, Nadiya Akmal and Zaidi, Kamilatulhusna and Salleh, Baharuddin and Azuddin, Nurul Farizah and Abdul Aziz, Fashli Aziz and Munusamy, Umaiyal and Othman, Rofina Yasmin (2019) Standardized bioassays: An improved method for studying Fusarium oxysporum f. sp. cubense race 4 (FocR4) pathogen stress response in musa acuminata cv. ‘Berangan’. Asia Pacific Journal of Molecular Biology and Biotechnology, 27 (3). pp. 101-112. ISSN 0128-7451

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Abstract

To date, there is no standardized Fusarium bioassay protocol established owing partly to the wide variety of Fusarium oxysporum f. sp. cubense (Foc) isolates and banana cultivars present. Thus, validation of the infection parameters is deemed essential prior to each bioassay experiment. In the current study, a simple standardized workflow was developed based on available assays for testing Fusarium wilt disease response in Musa acuminata using M. acuminata cv. ‘Berangan’ of tissue-culture origin as a model. The phenotypic assays were able to detect external disease symptoms less than one week post-inoculation, while the molecular approach using RT-qPCR identified differential expression of catalase (CAT), pathogenesis-related 10 (PR10), phenylalanine ammonia-lyase (PAL) and xylanase (XYL) genes as early as day 0. The transcript levels of PR10 and XYL fluctuated over 4 days of Foc Race 4 (FocR4 C1 HIR isolate) infection while the expression of CAT steadily increased over time. In contrast, PAL was highly upregulated at 2 days post-inoculation. These signature changes suggest that all genes tested might be involved in the early defense response of ‘Berangan’ plants against FocR4 infection. ‘Berangan’ cultivar was found to be highly susceptible to Foc Race 4 (C1 HIR isolate) with leaf symptoms index (LSI) and rhizome discoloration index (RDI) scores of 4.257 and 5.971, respectively. The procedure elaborated in this study can be used as a reference Foc bioassay for reproducible and comparable results possibly across cultivars and test isolates due to its simple steps aided by integration of phenotypic and molecular approach. © 2019, University of Malaya. All rights reserved.

Item Type: Article
Uncontrolled Keywords: Banana; Foc bioassay; Fusarium oxysporum f. sp. cubense; Fusarium wilt; Musa acuminata cv. ‘Berangan’; Plant microbe interaction
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history
Divisions: Centre for Foundation Studies in Science > Biology Division
Faculty of Science > Institute of Biological Sciences
Deputy Vice Chancellor (Research & Innovation) Office > Centre for Research in Biotechnology for Agriculture
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 10 Mar 2020 03:25
Last Modified: 10 Mar 2020 03:25
URI: http://eprints.um.edu.my/id/eprint/23976

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