Nam, Hui Yin and Pingguan-Murphy, Belinda and Abbas, Azlina Amir and Merican, Azhar Mahmood and Kamarul, Tunku (2019) Uniaxial Cyclic Tensile Stretching at 8% Strain Exclusively Promotes Tenogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stromal Cells. Stem Cells International, 2019. pp. 1-16. ISSN 1687-966X, DOI https://doi.org/10.1155/2019/9723025.
Full text not available from this repository.Abstract
The present study was conducted to establish the amount of mechanical strain (uniaxial cyclic stretching) required to provide optimal tenogenic differentiation expression in human mesenchymal stromal cells (hMSCs) in vitro, in view of its potential application for tendon maintenance and regeneration. Methods. In the present study, hMSCs were subjected to 1 Hz uniaxial cyclic stretching for 6, 24, 48, and 72 hours; and were compared to unstretched cells. Changes in cell morphology were observed under light and atomic force microscopy. The tenogenic, osteogenic, adipogenic, and chondrogenic differentiation potential of hMSCs were evaluated using biochemical assays, extracellular matrix expressions, and selected mesenchyme gene expression markers; and were compared to primary tenocytes. Results. Cells subjected to loading displayed cytoskeletal coarsening, longer actin stress fiber, and higher cell stiffness as early as 6 hours. At 8% and 12% strains, an increase in collagen I, collagen III, fibronectin, and N-cadherin production was observed. Tenogenic gene expressions were highly expressed (p<0.05) at 8% (highest) and 12%, both comparable to tenocytes. In contrast, the osteoblastic, chondrogenic, and adipogenic marker genes appeared to be downregulated. Conclusion. Our study suggests that mechanical loading at 8% strain and 1 Hz provides exclusive tenogenic differentiation; and produced comparable protein and gene expression to primary tenocytes. © 2019 Hui Yin Nam et al.
| Item Type: | Article |
|---|---|
| Funders: | HIR-Ministry of Education Grant (reference number: UM.C/625/1/HIR/MOHE/-CHAN/03, account number: A000003–50001) and UMRG (grant number RP005E-13HTM) |
| Uncontrolled Keywords: | Tendons; Stem Cells; Tenogenic differentiation |
| Subjects: | R Medicine |
| Divisions: | Faculty of Engineering Faculty of Medicine |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 28 Feb 2020 00:57 |
| Last Modified: | 28 Feb 2020 00:57 |
| URI: | http://eprints.um.edu.my/id/eprint/23893 |
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