Inherent differential propensity of dental pulp stem cells derived from human deciduous and permanent teeth

Zain, R.B.; Govindasamy, V.; Abdullah, A.N.; Ronald, V.S.; Musa, S.; Aziz, Z.A.C; Totey, S.; Bhonde, R.R.; Kasim, N.H. (2010) Inherent differential propensity of dental pulp stem cells derived from human deciduous and permanent teeth. Journal of Endodontics, 36 (9). pp. 1504-1515. ISSN 0099-2399

[img]
Preview
PDF [error in script]
Download (18Kb) | Preview
    [img]
    Preview
    PDF [error in script]
    Download (2527Kb) | Preview

      Abstract

      Introduction: Lately, several new stem cell sources and their effective isolation have been reported that claim to have potential for therapeutic applications. However, it is not yet clear which type of stem cell sources are most potent and best for targeted therapy. Lack of understanding of nature of these cells and their lineagespecific propensity might hinder their full potential. Therefore, understanding the gene expression profile that indicates their lineage-specific proclivity is fundamental to the development of successful cell-based therapies. Methods: We compared proliferation rate, gene expression profile, and lineage-specific propensity of stem cells derived from human deciduous (SCD) and permanent teeth (DPSCs) over 5 passages. Results: The proliferation rate of SCD was higher (cell number, 25 × 106 cells/mL; percent colony-forming units [CFUs], 151.67 ± 10.5; percent cells in S/G2 phase, 12.4 ± 1.48) than that of DPSCs (cell number, 21 × 106 cells/mL; percent CFUs, 133 ± 17.62; percent cells in S/G2 phase, 10.4 ± 1.18). It was observed that fold expression of several pluripotent markers such as OCT4, SOX2, NANOG, and REX1 were higher (>2) in SCD as compared with DPSCs. However, DPSCs showed higher expression of neuroectodermal markers PAX6, GBX2, and nestin (fold expression >100). Similarly, higher neurosphere formation and neuronal marker expression (NF, GFAP) were found in the differentiated DPSCs into neuron-like cells as compared with SCD. Conclusions: This study thus demonstrates that both SCD and DPSCs exhibit specific gene expression profile, with clear-cut inclination of DPSCs toward neuronal lineage. Copyright © 2010 American Association of Endodontists.

      Item Type: Article
      Creators:
      1. Zain, R.B.(Univ Malaya, Fac Dent, Dept Oral Pathol Oral Med& Periodontol, OCRCC, Kuala Lumpur 50603, Malaysia )
      2. Govindasamy, V.(Stempeut Res Malaysia Sdn Bhd, Kuala Lumpur, Malaysia)
      3. Abdullah, A.N.(Univ Malaya, Fac Dent, Dept Childrens Dent & Orthodont, Kuala Lumpur 50603, Malaysia )
      4. Ronald, V.S.(Univ Malaya, Fac Dent, Dept Childrens Dent & Orthodont, Kuala Lumpur 50603, Malaysia )
      5. Musa, S.(Univ Malaya, Fac Dent, Dept Childrens Dent & Orthodont, Kuala Lumpur 50603, Malaysia )
      6. Aziz, Z.A.C(Univ Malaya, Fac Dent, Dept Conservat Dent, Kuala Lumpur 50603, Malaysia )
      7. Totey, S.(Stempeut Res Malaysia Sdn Bhd, Kuala Lumpur, Malaysia)
      8. Bhonde, R.R.(Stempeut Res Malaysia Sdn Bhd, Kuala Lumpur, Malaysia)
      9. Kasim, N.H.(Univ Malaya, Fac Dent, Dept Conservat Dent, Kuala Lumpur 50603, Malaysia )
      Journal or Publication Title: Journal of Endodontics
      Additional Information: Address requests for reprints to Noor Hayaty Abu Kasim, BDS, PhD, Department of Conservative Dentistry, Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, Malaysia. E-mail address: nhayaty@um.edu.my.
      Uncontrolled Keywords: In-Vitro; Stromal Cells; Marrow; Vivo; Dentinogenesis; Pluripotency; Neurogenesis; Generation; Population; Expression
      Subjects: R Medicine
      R Medicine > RK Dentistry
      R Medicine > RK Dentistry > Oral surger
      Divisions: Academy of Islamic Studies > Dept of Applied Sciences with Islamic Studies
      Depositing User: Prof. Dr. Rosnah Mohd Zain
      Date Deposited: 10 Jan 2012 10:33
      Last Modified: 11 Dec 2013 10:49
      URI: http://eprints.um.edu.my/id/eprint/2371

      Actions (For repository staff only: Login required)

      View Item

      Document Downloads

      More statistics for this item...