Inherent differential propensity of dental pulp stem cells derived from human deciduous and permanent teeth

Zain, R.B. and Govindasamy, V. and Abdullah, A.N. and Ronald, V.S. and Musa, S. and Che Ab Aziz, Zeti Adura and Totey, S. and Bhonde, R.R. and Kasim, N.H. (2010) Inherent differential propensity of dental pulp stem cells derived from human deciduous and permanent teeth. Journal of Endodontics, 36 (9). pp. 1504-1515. ISSN 0099-2399

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Abstract

Introduction: Lately, several new stem cell sources and their effective isolation have been reported that claim to have potential for therapeutic applications. However, it is not yet clear which type of stem cell sources are most potent and best for targeted therapy. Lack of understanding of nature of these cells and their lineagespecific propensity might hinder their full potential. Therefore, understanding the gene expression profile that indicates their lineage-specific proclivity is fundamental to the development of successful cell-based therapies. Methods: We compared proliferation rate, gene expression profile, and lineage-specific propensity of stem cells derived from human deciduous (SCD) and permanent teeth (DPSCs) over 5 passages. Results: The proliferation rate of SCD was higher (cell number, 25 × 106 cells/mL; percent colony-forming units [CFUs], 151.67 ± 10.5; percent cells in S/G2 phase, 12.4 ± 1.48) than that of DPSCs (cell number, 21 × 106 cells/mL; percent CFUs, 133 ± 17.62; percent cells in S/G2 phase, 10.4 ± 1.18). It was observed that fold expression of several pluripotent markers such as OCT4, SOX2, NANOG, and REX1 were higher (>2) in SCD as compared with DPSCs. However, DPSCs showed higher expression of neuroectodermal markers PAX6, GBX2, and nestin (fold expression >100). Similarly, higher neurosphere formation and neuronal marker expression (NF, GFAP) were found in the differentiated DPSCs into neuron-like cells as compared with SCD. Conclusions: This study thus demonstrates that both SCD and DPSCs exhibit specific gene expression profile, with clear-cut inclination of DPSCs toward neuronal lineage. Copyright © 2010 American Association of Endodontists.

Item Type: Article
Additional Information: Address requests for reprints to Noor Hayaty Abu Kasim, BDS, PhD, Department of Conservative Dentistry, Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, Malaysia. E-mail address: nhayaty@um.edu.my.
Uncontrolled Keywords: In-Vitro; Stromal Cells; Marrow; Vivo; Dentinogenesis; Pluripotency; Neurogenesis; Generation; Population; Expression
Subjects: R Medicine
R Medicine > RK Dentistry
R Medicine > RK Dentistry > Oral surger
Divisions: Academy of Islamic Studies > Dept of Applied Sciences with Islamic Studies
Depositing User: Prof. Dr. Rosnah Mohd Zain
Date Deposited: 10 Jan 2012 02:33
Last Modified: 10 Oct 2018 14:00
URI: http://eprints.um.edu.my/id/eprint/2371

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