Exploring ligand-protein interaction: A laboratory exercise on herbicide binding to plasma transport protein

Roslan, Amira Adlin and Tayyab, Saad (2019) Exploring ligand-protein interaction: A laboratory exercise on herbicide binding to plasma transport protein. Biochemistry and Molecular Biology Education, 47 (2). pp. 156-160. ISSN 1470-8175

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Official URL: https://doi.org/10.1002/bmb.21207

Abstract

A laboratory exercise on the interaction between the herbicide pendimethalin (PM) and goat serum albumin (GSA), a carrier protein present in mammalian blood circulation, is described. Fluorescence spectroscopy was used to study the binding reaction between PM and GSA. Titration of a constant amount of the protein (GSA) with increasing ligand (PM) concentrations produced a consecutive decrease in the protein's fluorescence. Treatment of the fluorescence quenching data according to the Stern–Volmer equation yielded the values of the Stern–Volmer constant (K sv ) and bimolecular quenching rate constant (k q ), whereas values of the binding constant (K a ) and number of binding sites (n) were obtained from the double logarithmic plot. This experiment provides an exciting opportunity for undergraduate students to independently perform ligand binding studies with a protein, in addition to providing the means for the determination of their binding parameters. © 2019 International Union of Biochemistry and Molecular Biology, 47(2): 156–160, 2019. © 2019 International Union of Biochemistry and Molecular Biology

Item Type: Article
Uncontrolled Keywords: fluorescence spectroscopy; goat serum albumin; ligand-protein interaction; Pendimethalin
Subjects: Q Science > Q Science (General)
Q Science > QD Chemistry
Q Science > QH Natural history
Q Science > QR Microbiology
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 03 Feb 2020 02:07
Last Modified: 03 Feb 2020 02:07
URI: http://eprints.um.edu.my/id/eprint/23634

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