Gene expression in human oral squamous cell carcinoma is influenced by risk factor exposure

Zain, R.B.; Gutkind, J.S; Teo, S.H.; Cheong, S.C.; Chandramouli, G.V.; Saleh, A.; Lau, S.H.; Sivakumaren, S.; Pathmanathan, R.; Prime, S.S.; Patel, V. (2009) Gene expression in human oral squamous cell carcinoma is influenced by risk factor exposure. Oral Oncology, 45 (8). pp. 712-719. ISSN 1368-8375

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      Abstract

      Oral squamous cell carcinoma (OSCC) is a world health problem and is associated with exposure to different risk factors. In the west, smoking and alcohol consumption are considered to be the main risk factors whilst in India and southeast Asia, betel quid (BQ) chewing is predominant. In this study, we compared the gene expression patterns of oral cancers associated with BQ chewing to those caused by smoking using Affymetrix microarrays. We found that 281 genes were differentially expressed between OSCC and normal oral mucosa regardless of aetiological factors including MMP1, PLAU, MAGE-D4, GNA12, IFITM3 and NMU. Further, we identified 168 genes that were differentially expressed between the BQ and smoking groups including CXCL-9, TMPRSS2, CA12 and RNF24. The expression of these genes was validated using qPCR using independent tissue samples. The results demonstrate that whilst common genes/pathways contribute to the development of oral cancer, there are also other gene expression changes that are specific to certain risk factors. The findings suggest that different carcinogens activate or inhibit specific pathways during cancer development and progression. These unique gene expression profiles should be taken into consideration when developing biomarkers for future use in prognostic or therapeutic applications. © 2008 Elsevier Ltd.

      Item Type: Article
      Creators:
      1. Zain, R.B.(Oral Cancer Research and Co-ordinating Centre, University of Malaya, Kuala Lumpur, Malaysia)
      2. Gutkind, J.S(Oral and Pharyngeal Cancer Branch, National Institute of Craniofacial and Dental Research, National Institutes of Health, Bethesda, MD 20892, USA)
      3. Teo, S.H.(Cancer Research Initiatives Foundation (CARIF), 2nd Floor Outpatient Centre, Subang Jaya Medical Centre, 47500 Subang Jaya, Selangor, Malaysia)
      4. Cheong, S.C.(Cancer Research Initiatives Foundation (CARIF), 2nd Floor Outpatient Centre, Subang Jaya Medical Centre, 47500 Subang Jaya, Selangor, Malaysia)
      5. Chandramouli, G.V.(Laboratory of Biosystems and Cancer, National Institutes of Health, Bethesda, MD 20892, USA )
      6. Saleh, A.(Cancer Research Initiatives Foundation (CARIF), 2nd Floor Outpatient Centre, Subang Jaya Medical Centre, 47500 Subang Jaya, Selangor, Malaysia)
      7. Lau, S.H.(Stomatology Unit, Institute for Medical Research, Jalan Pahang, 50588 Kuala Lumpur, Malaysia)
      8. Sivakumaren, S.(Pathology Department, Diagnostic Laboratory, Subang Jaya Medical Centre, 47500 Subang Jaya, Selangor, Malaysia)
      9. Pathmanathan, R.(Pathology Department, Diagnostic Laboratory, Subang Jaya Medical Centre, 47500 Subang Jaya, Selangor, Malaysia)
      10. Prime, S.S.(Department of Oral and Dental Science, University of Bristol, United Kingdom)
      11. Patel, V.(Oral and Pharyngeal Cancer Branch, National Institute of Craniofacial and Dental Research, National Institutes of Health, Bethesda, MD 20892, USA)
      Journal or Publication Title: Oral Oncology
      Additional Information: Oral Cancer Research and Co-ordinating Centre, University of Malaya, Kuala Lumpur, Malaysia
      Uncontrolled Keywords: Oral squamous cell carcinoma; Gene expression; Betel quid; Smoking; Risk factors
      Subjects: R Medicine
      R Medicine > RK Dentistry
      Divisions: Faculty of Dentistry > Dept of Oral Pathology & Oral Medicine & Periodontology
      Depositing User: Prof. Dr. Rosnah Mohd Zain
      Date Deposited: 06 Jan 2012 09:07
      Last Modified: 11 Dec 2013 14:58
      URI: http://eprints.um.edu.my/id/eprint/2353

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