Tang, Esther Lai Har and Tan, Nget Hong and Fung, Shin Yee and Tan, Choo Hock (2019) Comparative proteomes, immunoreactivities and neutralization of procoagulant activities of Calloselasma rhodostoma (Malayan pit viper) venoms from four regions in Southeast Asia. Toxicon, 169. pp. 91-102. ISSN 0041-0101, DOI https://doi.org/10.1016/j.toxicon.2019.08.004.
Full text not available from this repository.Abstract
The intraspecific geographical venom variations of Calloselasma rhodostoma from Malaysia (CR-M), Indonesia (CR-I), Thailand (CR-T) and Vietnam (CR-V) were investigated through 1D SDS-PAGE and nano-ESI-LCMS/MS. The venom antigenicity, procoagulant activities and neutralization using Thai C. rhodostoma Monovalent Antivenom (CRMAV) were also investigated. SDS-PAGE patterns of the venoms were relatively similar with minor variations. Proteomic analysis revealed that snake venom metalloproteinases (SVMPs, particularly P–I class), serine proteases (SVSPs) and snaclecs dominated the venom protein composition (68.96–81.80%), followed by L-amino acid oxidase (LAAO) and phospholipase A2 (PLA2) (7.37–11.08% and 5.18–13.81%, respectively), corroborating C. rhodostoma envenoming effects (hemorrhage, consumptive coagulopathy, thrombocytopenia and local tissue necrosis). Other proteins of lower abundances (2.82–9.13%) identified include cysteine-rich secretory proteins (CRISP), phospholipase B, phosphodiesterase, nerve growth factor, 5′-nucleotidase, aminopeptidase and hyaluronidase. All four venoms exhibited strong procoagulant effects which were neutralized by CRMAV to different extents. CRMAV immunoreactivity was high toward venoms of CR-M, CR-I and CR-T but relatively low for CR-V venom. Among the venom samples from different locales, CR-V venom proteome has the smallest SVMP composition while SVSP, PLA2 and phosphodiesterase were more abundant in the venom. These variations in C. rhodostoma venom protein composition could partly explain the differences seen in immunoreactivity. (198 words). © 2019
| Item Type: | Article |
|---|---|
| Funders: | Fundamental Research Grant Scheme ( FP028-2014A ) from the Government of Malaysia, Research grant ( RF007C-2018 ) from the University of Malaya |
| Uncontrolled Keywords: | Venomics; Antivenom; Immunoreactivity; Antigenicity; Malayan pit viper |
| Subjects: | R Medicine |
| Divisions: | Faculty of Medicine |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 23 Dec 2019 03:37 |
| Last Modified: | 23 Dec 2019 03:37 |
| URI: | http://eprints.um.edu.my/id/eprint/23282 |
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