The Effect of Pathogenesis-Related 10 (PR-10) Gene on the Progression of Fusarium Wilt in Musa acuminata cv. Berangan

Baharum, Nadiya Akmal and Othman, Rofina Yasmin and Mohd-Yusuf, Yusmin and Tan, Boon Chin and Zaidi, Kamilatulhusna and Khalid, Norzulaani (2018) The Effect of Pathogenesis-Related 10 (PR-10) Gene on the Progression of Fusarium Wilt in Musa acuminata cv. Berangan. Sains Malaysiana, 47 (10). pp. 2291-2300. ISSN 0126-6039

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Official URL: https://doi.org/10.17576/jsm-2018-4710-05

Abstract

PR-10 is a member of pathogenesis-related (PR) genes elicited by the plant’s defense mechanism during pathogen attack. Elevated expression of PR-10 upon different pathogen invasions has been observed in many plant species suggesting its role as an anti-bacterial, anti-viral and anti-fungal gene. However, the effect of PR-10 in mitigating the infection of Fusarium oxysporum f. sp. cubense (Foc), the causal agent of Fusarium wilt in banana has not been reported. In this study, the coding sequences of PR-10 gene isolated from Foc resistant Musa acuminata ssp. malaccensis (MaPR-10) were integrated into a local Foc susceptible commercial banana cultivar, Berangan via co-cultivation of embryogenic cell suspension and Agrobacterium tumefaciens. Out of 17 putative transgenic lines established, 11 of them positively harbored MaPR-10. Among these, Line-19 plantlets showed the most rapid in-vitro propagation and successfully over-expressed the transgene. Following a nursery challenge experiment with a virulent Foc race 4 (CI HIR) isolate, about 30% of Line-19 plants showed a one-week delay in disease progression when compared to the untransformed controls. From the final evaluation performed in the 5th week-post-inoculation, the leaf symptoms index (LSI) and rhizome discoloration index (RDI) of Line-19 was 3.4 and 6.1, respectively, indicating the disease had progressed. The findings of this study enrich the current existing knowledge on the roles of PR-10 in combating fungal disease in plants.

Item Type: Article
Uncontrolled Keywords: Fusarium oxysporum f. sp. cubense; Fusarium wilt; Pathogenesis-related protein; PR-10; Transgenic banana
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history
Divisions: Centre for Foundation Studies in Science > Biology Division
Faculty of Science > Institute of Biological Sciences
Deputy Vice Chancellor (Research & Innovation) Office > Centre for Research in Biotechnology for Agriculture
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 17 Sep 2019 01:41
Last Modified: 17 Sep 2019 01:41
URI: http://eprints.um.edu.my/id/eprint/22370

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