Baharum, Nadiya Akmal and Othman, Rofina Yasmin and Mohd-Yusuf, Yusmin and Tan, Boon Chin and Zaidi, Kamilatulhusna and Khalid, Norzulaani (2018) The Effect of Pathogenesis-Related 10 (PR-10) Gene on the Progression of Fusarium Wilt in Musa acuminata cv. Berangan. Sains Malaysiana, 47 (10). pp. 2291-2300. ISSN 0126-6039, DOI https://doi.org/10.17576/jsm-2018-4710-05.
Full text not available from this repository.Abstract
PR-10 is a member of pathogenesis-related (PR) genes elicited by the plant’s defense mechanism during pathogen attack. Elevated expression of PR-10 upon different pathogen invasions has been observed in many plant species suggesting its role as an anti-bacterial, anti-viral and anti-fungal gene. However, the effect of PR-10 in mitigating the infection of Fusarium oxysporum f. sp. cubense (Foc), the causal agent of Fusarium wilt in banana has not been reported. In this study, the coding sequences of PR-10 gene isolated from Foc resistant Musa acuminata ssp. malaccensis (MaPR-10) were integrated into a local Foc susceptible commercial banana cultivar, Berangan via co-cultivation of embryogenic cell suspension and Agrobacterium tumefaciens. Out of 17 putative transgenic lines established, 11 of them positively harbored MaPR-10. Among these, Line-19 plantlets showed the most rapid in-vitro propagation and successfully over-expressed the transgene. Following a nursery challenge experiment with a virulent Foc race 4 (CI HIR) isolate, about 30% of Line-19 plants showed a one-week delay in disease progression when compared to the untransformed controls. From the final evaluation performed in the 5th week-post-inoculation, the leaf symptoms index (LSI) and rhizome discoloration index (RDI) of Line-19 was 3.4 and 6.1, respectively, indicating the disease had progressed. The findings of this study enrich the current existing knowledge on the roles of PR-10 in combating fungal disease in plants.
| Item Type: | Article |
|---|---|
| Funders: | Grant (53-02-03-1068) financed by the Ministry of Science, Technology and Innovation (MOSTI) Malaysia, University of Malaya Research Grant (RP005C-13BIO), University Malaya High Impact Research Grant (UM.C/625/1/HIR/MOHE/SCI/18), CEBAR Research University Grant (RU006-2017) |
| Uncontrolled Keywords: | Fusarium oxysporum f. sp. cubense; Fusarium wilt; Pathogenesis-related protein; PR-10; Transgenic banana |
| Subjects: | Q Science > Q Science (General) Q Science > QH Natural history |
| Divisions: | Centre for Foundation Studies in Science > Biology Division Faculty of Science > Institute of Biological Sciences Deputy Vice Chancellor (Research & Innovation) Office > Centre for Research in Biotechnology for Agriculture |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 17 Sep 2019 01:41 |
| Last Modified: | 17 Sep 2019 01:41 |
| URI: | http://eprints.um.edu.my/id/eprint/22370 |
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