Bonny, Sharmin Quazi and Hossain, M.A. Motalib and Thong, Kwai Lin and Ali, Md. Eaqub (2018) Multiplex MPN-PCR for the enumeration of three major Vibrios in raw fishes in Malaysia. Food Control, 90. pp. 459-465. ISSN 0956-7135, DOI https://doi.org/10.1016/j.foodcont.2018.02.034.
Full text not available from this repository.Abstract
Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus are the most notorious fish borne-pathogens responsible for several deadly diseases in human. However, the prevalence of these three pathogenic vibrios in Malaysian fishes has not been thoroughly investigated, leaving a study gap for the tracing of possible Vibrio outbreak through carrier fish consumption. The most-probable-number-PCR (MPN-PCR) assays are highly sensitive for the identification of a single bacterial pathogen but they have not been applied for the discrimination of major vibrios in a multiplex platform. This study coupled MPN method with Multiplex PCR to differentiate three major vibrios in a one test platform. A total of 120 fish samples were analyzed and the prevalence of V. parahaemolyticus, V. cholerae, and V. vulnificus was 48.33%, 27%, and 32.50%, respectively. When simplex MPN-PCR was applied similar results were obtained at P < 0.05, validating the accuracy of multiplex MPN-PCR. The microbial loads of V. parahaemolyticus, V. cholerae, and V. vulnificus in the tested samples were 0–2.43 × 106, 0–2.16 × 106, and 0–4.93 × 105 MPN g−1, respectively. Primers based on housekeeping pntA gene amplified V. parahaemolyticus (409 bp) and V. cholerae (338 bp) but virulent vvhA gene detected V. vulnificus (205 bp). Thus the 27–48% prevalence of targeted vibrios in consumable fishes indicated there is considerable risk of Vibrio onset in Malaysia due to the consumption of undercooked or raw fish products.
| Item Type: | Article |
|---|---|
| Funders: | University of Malaya Research Grant No. GC001A-14SBS and PG245-2016A |
| Uncontrolled Keywords: | Multiplex MPN-PCR assay; Fish-borne pathogens; Zoonotic vibrios; Microbial load; Raw and undercooked fish products |
| Subjects: | Q Science > Q Science (General) Q Science > QH Natural history |
| Divisions: | Faculty of Science > Institute of Biological Sciences Deputy Vice Chancellor (Research & Innovation) Office > Centre for Research in Biotechnology for Agriculture Deputy Vice Chancellor (Research & Innovation) Office > Nanotechnology & Catalysis Research Centre |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 11 Sep 2019 09:04 |
| Last Modified: | 11 Sep 2019 09:04 |
| URI: | http://eprints.um.edu.my/id/eprint/22302 |
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