DNA barcoding relates Trichuris species from a human and a man’s best friend to non-human primate sources

Brandon-Mong, Guo Jie and Ketzis, Jennifer K. and Choy, J.S. and Boonroumkaew, Patcharaporn and Mahboob, Tooba and Sawangjaroen, Nongyao and Yasiri, Atipat and Janwan, Penchom and Tan, Tian Chye and Nissapatorn, Veeranoot (2018) DNA barcoding relates Trichuris species from a human and a man’s best friend to non-human primate sources. Tropical Biomedicine, 35 (4). pp. 1131-1139. ISSN 0127-5720

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Official URL: http://msptm.org/files/Vol35No4/1131-1139-Veeranoo...

Abstract

Trichuris trichiura, the whipworm of humans, is one of the most prevalent soil-transmitted helminths (STH) reported worldwide. According to a recent study, out of 289 STH studies in Southeast Asia, only three studies used molecular methods. Hence, the genetic assemblages of Trichuris in Southeast Asia are poorly understood. In this study, we used partial mitochondrial DNA (cytochrome c oxidase subunit 1 or COI) sequences for analysis. Trichuris grouped in a same clade with different hosts indicate the potential of cross infection between hosts. Based on COI, the adult Trichuris isolated from a Malaysian patient was most closely related to Trichuris isolated from Papio anubis (olive baboons) from the USA. The Trichuris isolated from the dog from Malaysia was genetically similar to a Trichuris species isolated from Macaca silenus (lion-tailed macaque) from Czech Republic. Both the human and dog isolated Trichuris grouped in clades with different hosts indicating the potential of cross infection between hosts. Specific PCR primers based on the partial COI of T. trichiura isolated from African green monkey and T. serrata were designed and successfully amplified using multiplex PCR of the pooled DNA samples. Our results suggest a complex parasite-host relationship, and support the theory of cross infection of Trichuris between humans and non-human primates as suggested in previous publications.

Item Type: Article
Uncontrolled Keywords: Nematoda; Trichuris; Host species
Subjects: R Medicine
Divisions: Faculty of Medicine
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 11 Sep 2019 05:05
Last Modified: 11 Sep 2019 05:05
URI: http://eprints.um.edu.my/id/eprint/22296

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