Genetic and haplotype analyses targeting cytochrome b gene of Plasmodium knowlesi isolates of Malaysian Borneo and Peninsular Malaysia

Chong, Eric Tzyy Jiann and Neoh, Joveen Wan Fen and Lau, Tiek Ying and Lim, Yvonne Ai Lian and Chua, Kek Heng and Lee, Ping Chin (2018) Genetic and haplotype analyses targeting cytochrome b gene of Plasmodium knowlesi isolates of Malaysian Borneo and Peninsular Malaysia. Acta Tropica, 181. pp. 35-39. ISSN 0001-706X

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Official URL: https://doi.org/10.1016/j.actatropica.2018.01.018

Abstract

Malaria is a notorious disease which causes major global morbidity and mortality. This study aims to investigate the genetic and haplotype differences of Plasmodium knowlesi (P. knowlesi) isolates in Malaysian Borneo and Peninsular Malaysia based on the molecular analysis of the cytochrome b (cyt b) gene. The cyt b gene of 49 P. knowlesi isolates collected from Sabah, Malaysian Borneo and Peninsular Malaysia was amplified using PCR, cloned into a commercialized vector and sequenced. In addition, 45 cyt b sequences were retrieved from humans and macaques bringing to a total of 94 cyt b gene nucleotide sequences for phylogenetic analysis. Genetic and haplotype analyses of the cyt b were analyzed using MEGA6 and DnaSP ver. 5.10.01. The haplotype genealogical linkage of cyt b was generated using NETWORK ver. 4.6.1.3. Our phylogenetic tree revealed the conservation of the cyt b coding sequences with no distinct cluster across different geographic regions. Nucleotide analysis of cyt b showed that the P. knowlesi isolates underwent purifying selection with population expansion, which was further supported by extensive haplotype sharing between the macaques and humans from Malaysian Borneo and Peninsular Malaysia in the median-joining network analysis. This study expands knowledge on conservation of the zoonotic P. knowlesi cyt b gene between Malaysian Borneo and Peninsular Malaysia.

Item Type: Article
Uncontrolled Keywords: Plasmodium knowlesi; Cytochrome b; Malaysian borneo; Peninsular Malaysia; Genetic diversity; Haplotype
Subjects: R Medicine
Divisions: Faculty of Medicine
Centre of Excellence for Research in AIDS (CERiA)
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 04 Sep 2019 04:32
Last Modified: 04 Sep 2019 04:32
URI: http://eprints.um.edu.my/id/eprint/22209

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