Factors Influencing the Successful Isolation and Expansion of Aging Human Mesenchymal Stem Cells

Chong, Pan Pan and Selvaratnam, Lakshmi and Abbas, Azlina Amir and Kamarul, Tunku (2018) Factors Influencing the Successful Isolation and Expansion of Aging Human Mesenchymal Stem Cells. Open Life Sciences, 13 (1). pp. 279-284. ISSN 2391-5412

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Official URL: https://doi.org/10.1515/biol-2018-0034

Abstract

Most studies highlight mesenchymal stem cells (MSCs) extracted primarily from bone marrow (BM), very few report the use of peripheral blood (PB), often due to the associated low seeding density and difficulties with extraction techniques. As ageing populations are becoming more predominant globally, together with escalating demands for MSC transplantation and tissue regeneration, obtaining quality MSCs suitable for induced differentiation and biological therapies becomes increasingly important. In this study, BM and PB were obtained from elderly patients and extracted MSCs grown in vitro to determine their successful isolation and expansion. Patients' socio-demographic background and other medical information were obtained from medical records. Successful and failed cultures were correlated with key demographic and medical parameters. A total of 112 samples (BM or PB) were used for this study. Of these, 50 samples (44.6%) were successfully cultured according to standardised criteria with no signs of contamination. Our comparative analyses demonstrated no statistical correlation between successful MSC cultures and any of the six demographic or medical parameters examined, including sample quantity, age, sex, race, habits and underlying comorbidities of sample donors. In conclusion, the present study demonstrates that typical demographics and comorbidities do not influence successful MSC isolation and expansion in culture.

Item Type: Article
Uncontrolled Keywords: Mesenchymal stem cells; Ageing, Bone marrow; Peripheral blood; Demographic factors; Comorbidities; Contamination; Mononuclear cells; Density gradient centrifugation
Subjects: R Medicine
Divisions: Faculty of Medicine
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 04 Sep 2019 03:52
Last Modified: 04 Sep 2019 03:52
URI: http://eprints.um.edu.my/id/eprint/22202

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