Foo, Yiing Yee and Kabir, Md Zahirul and Periasamy, Vengadesh and Malek, Sri Nurestri Abd and Tayyab, Saad (2018) Spectroscopic studies on the interaction of green synthesized-gold nanoparticles with human serum albumin. Journal of Molecular Liquids, 265. pp. 105-113. ISSN 0167-7322, DOI https://doi.org/10.1016/j.molliq.2018.05.115.
Full text not available from this repository.Abstract
The interaction of gold nanoparticles, synthesized using Curcuma mangga extract (CM-AuNPs) with human serum albumin (HSA) was investigated with the help of fluorescence, UV absorption and circular dichroism (CD) spectroscopy. In view of the positive correlation of Stern-Volmer constant with temperature, quenching of protein fluorescence observed upon addition of CM-AuNPs seems to occur through collisional mechanism. The quenching mechanism was further substantiated by UV absorption spectral results, where no significant change in the absorption spectrum of HSA was observed upon addition of CM-AuNPs. Analysis of the fluorescence quenching titration data revealed moderate binding affinity (Ka = 0.97 × 104 M−1 at 298 K) between CM-AuNP and HSA. The complexation between CM-AuNP and HSA was predicted to be stabilized by hydrophobic forces, as reflected from the thermodynamic data (ΔH = +35.5 kJ mol−1 and ΔS = +195.62 J mol−1 K−1). Three-dimensional fluorescence spectral analysis demonstrated perturbation of microenvironment around Trp and Tyr residues in HSA upon CM-AuNPs addition. While interaction between CM-AuNP and HSA produced significant tertiary structural change in the protein, secondary structures remained unaltered, as elucidated by near-UV and far-UV CD spectral analyses, respectively. ANS displacement experiments suggested Sudlow's Site II, located in subdomain IIIA, as the preferred binding site of CM-AuNP on HSA.
| Item Type: | Article |
|---|---|
| Funders: | High Impact Research (HIR) MoE Grant UM.C/625/1/HIR/MoE/SC/02 from the Ministry of Education Malaysia, PPP grant, PG199-2014B from the University of Malaya |
| Uncontrolled Keywords: | Nanoparticle-protein interaction; Fluorescence quenching; Curcuma mangga; Gold nanoparticles |
| Subjects: | Q Science > Q Science (General) Q Science > QC Physics Q Science > QH Natural history |
| Divisions: | Faculty of Science > Department of Physics Faculty of Science > Institute of Biological Sciences |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 26 Aug 2019 03:55 |
| Last Modified: | 26 Aug 2019 03:55 |
| URI: | http://eprints.um.edu.my/id/eprint/22064 |
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