Amino acid analysis of lipases from oil pollutant isolates: Cunninghamella verticillata and geotrichum candidum

Gopinath, Subash Chandra Bose and Anbu, Periasamy and Md Arshad, Mohd Khairuddin and Lakshmipriya, Thangavel and Voon, Chun Hong and Hashim, Uda and Al-Douri, Yarub and Citartan, Marimuthu and Tang, Thean-Hock and Chen, Yeng (2018) Amino acid analysis of lipases from oil pollutant isolates: Cunninghamella verticillata and geotrichum candidum. Songklanakarin Journal of Science and Technology, 40 (4). pp. 878-884. ISSN 0125-3395

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Abstract

Lipase is an enzyme commonly used in food, dairy, and other industries. Fungal lipases are more commonly used due to their secretion and easier production. Analyses of the amino acid composition of microbial lipases will hasten their potential usage in industrial applications. In this study, the major amino acid compositions of lipases secreted by oil pollutant isolates (Cunninghamella verticillata and Geotrichum candidum) enriched with fatty substances were analyzed by high performance liquid chromatography. Among eight major amino acids found in these lipases, histidine and ornithine were predominant. Lysine was absent from lipase generated by C. verticillata, while glutamine was absent from that produced by G. candidum. Conversely, glutamic acid, asparagine, histidine and arginine were present in slightly higher amounts in G. candidum. However, a slight decrease in aspartic acid and ornithine was observed in G. candidum. Analyses of the amino acids composition of lipase can potentially facilitate to predict the nature of this enzyme.

Item Type: Article
Uncontrolled Keywords: amino acid; Cunninghamella verticillata; Geotrichum candidum; high performance liquid chromatography; lipase
Subjects: Q Science > QC Physics
R Medicine > RK Dentistry
T Technology > TK Electrical engineering. Electronics Nuclear engineering
Divisions: Faculty of Dentistry
Deputy Vice Chancellor (Research & Innovation) Office > Nanotechnology & Catalysis Research Centre
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 21 Aug 2019 05:02
Last Modified: 21 Aug 2019 05:02
URI: http://eprints.um.edu.my/id/eprint/22004

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