Phenylpropanoids isolated from Piper sarmentosum Roxb. induce apoptosis in breast cancer cells through reactive oxygen species and mitochondrial-dependent pathways

Hematpoor, Arshia and Paydar, Mohammadjavad and Liew, Sook Yee and Sivasothy, Yasodha and Mohebali, Nooshin and Looi, Chung Yeng and Wong, Won Fen and Sofian-Azirun, Mohd and Awang, Khalijah (2018) Phenylpropanoids isolated from Piper sarmentosum Roxb. induce apoptosis in breast cancer cells through reactive oxygen species and mitochondrial-dependent pathways. Chemico-Biological Interactions, 279. pp. 210-218. ISSN 0009-2797

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Official URL: https://doi.org/10.1016/j.cbi.2017.11.014

Abstract

The aim of the present study is to isolate bioactive compounds from the roots of Piper sarmentosum and examine the mechanism of action using human breast cancer cell line (MDA-MB-231). Bioassay guided-fractionation of methanolic extract led to the isolation of asaricin (1) and isoasarone (2). Asaricin (1) and isoasarone (2) had significant cytotoxicity towards MDA-MB-231. MCF-10A (human normal breast epithelial cells) cells are less sensitive than MDA-MB-231, but they respond to the treatment with the same unit of measurement. Both compounds increase reactive oxygen species (ROS), decrease mitochondrial membrane potential (MMP) and enhance cytochrome c release in treated MDA-MB-231 cells. Isoasarone (2) markedly elevated caspase -8 and -3/7 activities and caused a decline in nuclear NF-κB translocation, suggesting extrinsic, death receptor-linked apoptosis pathway. Quantitative PCR results of MDA-MB-231 treated with asaricin (1) and isoasarone (2) showed altered expression of Bcl-2: Bax level. The inhibitory potency of these isolates may support the therapeutic uses of these compounds in breast cancer.

Item Type: Article
Uncontrolled Keywords: Cytotoxicity; Mitochondrial membrane; Piper sarmentosum; Phenylpropanoid
Subjects: Q Science > QD Chemistry
Q Science > QH Natural history
R Medicine
Divisions: Centre for Foundation Studies in Science > Chemistry Division
Faculty of Medicine
Faculty of Science > Dept of Chemistry
Faculty of Science > Institute of Biological Sciences
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 08 Aug 2019 06:03
Last Modified: 08 Aug 2019 06:03
URI: http://eprints.um.edu.my/id/eprint/21906

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