iTRAQ analysis of urinary proteins: Potential use of gelsolin and osteopontin to distinguish benign thyroid goiter from papillary thyroid carcinoma

Jayapalan, Jaime Jacqueline and Lee, Cheng Siang and Lee, Ching Chin and Ng, Khoon Leong and Junit, Sarni Mat and Hashim, Onn Haji (2018) iTRAQ analysis of urinary proteins: Potential use of gelsolin and osteopontin to distinguish benign thyroid goiter from papillary thyroid carcinoma. Clinical Biochemistry, 53. pp. 127-131. ISSN 0009-9120

Full text not available from this repository.
Official URL: https://doi.org/10.1016/j.clinbiochem.2018.01.008

Abstract

Background: Benign thyroid goiter (BTG) and papillary thyroid carcinoma (PTC) are often interchangeably misdiagnosed. Methods: Pooled urine samples of patients with BTG (n = 10), patients with PTC (n = 9) and healthy controls (n = 10) were subjected to iTRAQ analysis and immunoblotting. Results: The ITRAQ analysis of the urine samples detected 646 proteins, 18 of which showed significant altered levels (p < 0.01; fold-change > 1.5) between patients and controls. Whilst four urinary proteins were commonly altered in both BTG and PTC patients, 14 were unique to either BTG or PTC. Amongst these, four proteins were further chosen for validation using immunoblotting, and the enhanced levels of osteopontin in BTG patients and increased levels of a truncated gelsolin fragment in PTC patients, relative to controls, appeared to corroborate the findings of the iTRAQ analysis. Conclusion: The data of the present study is suggestive of the potential application of urinary osteopontin and gelsolin to discriminate patients with BTG from those with PTC non-invasively. However, this needs to be further validated in studies of individual urine samples.

Item Type: Article
Uncontrolled Keywords: Adult; Carcinoma, Papillary; Chromatography, Liquid; Female; Gelsolin; Goiter; Humans; Male; Mass Spectrometry; Middle Aged; Osteopontin; Thyroid Neoplasms
Subjects: R Medicine
Divisions: Faculty of Medicine
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 02 Aug 2019 02:00
Last Modified: 02 Aug 2019 02:00
URI: http://eprints.um.edu.my/id/eprint/21742

Actions (login required)

View Item View Item