Jayapalan, Jaime Jacqueline and Lee, Cheng Siang and Lee, Ching Chin and Ng, Khoon Leong and Junit, Sarni Mat and Hashim, Onn Haji (2018) iTRAQ analysis of urinary proteins: Potential use of gelsolin and osteopontin to distinguish benign thyroid goiter from papillary thyroid carcinoma. Clinical Biochemistry, 53. pp. 127-131. ISSN 0009-9120, DOI https://doi.org/10.1016/j.clinbiochem.2018.01.008.
Full text not available from this repository.Abstract
Background: Benign thyroid goiter (BTG) and papillary thyroid carcinoma (PTC) are often interchangeably misdiagnosed. Methods: Pooled urine samples of patients with BTG (n = 10), patients with PTC (n = 9) and healthy controls (n = 10) were subjected to iTRAQ analysis and immunoblotting. Results: The ITRAQ analysis of the urine samples detected 646 proteins, 18 of which showed significant altered levels (p < 0.01; fold-change > 1.5) between patients and controls. Whilst four urinary proteins were commonly altered in both BTG and PTC patients, 14 were unique to either BTG or PTC. Amongst these, four proteins were further chosen for validation using immunoblotting, and the enhanced levels of osteopontin in BTG patients and increased levels of a truncated gelsolin fragment in PTC patients, relative to controls, appeared to corroborate the findings of the iTRAQ analysis. Conclusion: The data of the present study is suggestive of the potential application of urinary osteopontin and gelsolin to discriminate patients with BTG from those with PTC non-invasively. However, this needs to be further validated in studies of individual urine samples.
| Item Type: | Article |
|---|---|
| Funders: | FRGS-2015-1( FP0032015A ) grant from the Ministry of Higher Education, HIR-MOHE H-2000100-E000009 grant from the University of Malaya |
| Uncontrolled Keywords: | Adult; Carcinoma, Papillary; Chromatography, Liquid; Female; Gelsolin; Goiter; Humans; Male; Mass Spectrometry; Middle Aged; Osteopontin; Thyroid Neoplasms |
| Subjects: | R Medicine |
| Divisions: | Faculty of Medicine |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 02 Aug 2019 02:00 |
| Last Modified: | 02 Aug 2019 02:00 |
| URI: | http://eprints.um.edu.my/id/eprint/21742 |
Actions (login required)
 |
View Item |
