Expression and DNA methylation of MET1, CMT3 and DRM2 during in vitro culture of Boesenbergia rotunda (L.) Mansf.

Karim, Rezaul and Tan, Yew Seong and Singh, Pooja and Nuruzzaman, Mohammed and Khalid, Norzulaani and Harikrishna, Jennifer Ann (2018) Expression and DNA methylation of MET1, CMT3 and DRM2 during in vitro culture of Boesenbergia rotunda (L.) Mansf. Philippine Agricultural Scientist, 101 (3). pp. 261-270. ISSN 0031-7454

Full text not available from this repository.
Official URL: https://pas.cafs.uplb.edu.ph/

Abstract

Somatic embryogenesis and plant regeneration are important developmental processes of in vitro culture, in which cells must undergo dedifferentiation, activation of cell division and reprograming of their metabolism, of their physiology and of their gene expression patterns. The processes of somatic embryogenesis and plant regeneration are also associated with changes in DNA methylation. In this study, the expression of METHYLTRANSFERASE 1, CHROMOMETHYLASE 3 and DOMAIN REARRANGED METHYLTRANSFERASE 2 was determined by Quantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR) while the DNA methylation level was determined by Bisulfite sequencing of plant cells from meristematic block, embryogenic and non-embryogenic calli, prolonged cell suspension culture, ex vitro leaf and in vitro leaf of regenerated plants of Boesenbergia rotunda. We observed that the expression of DNA methyltransferase genes METHYLTRANSFERASE 1, CHROMOMETHYLASE 3 and DOMAIN REARRANGED METHYLTRANSFERASE 2 was the highest in meristematic block followed by embryogenic callus, and the lowest was in watery callus. DNA methylation at CG, CHG and CHH sequence contexts was observed to be generally lower in embryogenic callus than in other samples. We observed relatively higher expression levels and lower levels of DNA methylation at CG, CHG and CHH sequence contexts of MET1, CMT3 and DRM2 associated with somatic embryogenesis and regenerability in Boesenbergia rotunda.

Item Type: Article
Uncontrolled Keywords: Bisulfite sequencing (BS-seq); Callus; DNA methylation; Gene expression; qRT-PCR; Somatic embryogenesis
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history
Divisions: Faculty of Science > Institute of Biological Sciences
Deputy Vice Chancellor (Research & Innovation) Office > Centre for Research in Biotechnology for Agriculture
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 22 Jul 2019 03:38
Last Modified: 22 Jul 2019 03:38
URI: http://eprints.um.edu.my/id/eprint/21678

Actions (login required)

View Item View Item