Micropropagation of nucellar embryos and their histological comparative study for regeneration ability with other explants of kinnow mandarin (Citrus reticulata blanco)

Kazmi, Syeda Kahkashan and Khan, Saifullah and Mirbahar, Ameer Ahmed and Kabir, Nurul (2018) Micropropagation of nucellar embryos and their histological comparative study for regeneration ability with other explants of kinnow mandarin (Citrus reticulata blanco). Pakistan Journal of Botany, 50 (1). pp. 345-353. ISSN 0556-3321

Full text not available from this repository.
Official URL: https://pdfs.semanticscholar.org/917a/d28627153bf2...

Abstract

Polyembryony is the most beneficial and distinct character in citrus seeds. This characteristic can be beneficial in citrus improvement programs. Nucellar embryos developed from nucellar wall in citrus seeds along with zygotic embryo are found to have high plants regeneration ability in comparison to zygotic embryos. Under In vitro culture conditions, nucellar embryos were detected and multiplied on MT media with 0.5ml of coconut water along with 50g/L sucrose. These nucellar embryonic tissues were also regenerated on MT media supplemented with 0.5 mg/L BAP and 0.5 mg/L kinetin. Many shoots were regenerated from nucellar embryonic tissue; these shoots were rooted on MT media supplemented with IBA 0.5mg/L and NAA 0.1 mg/L in addition to activated charcoal 0.5gm/L. The resulting plantlets were acclimatized in the green house. After micropropagation, histological studies of nucellar embryonic tissues were carried out under fluorescence microscope to examine their high regeneration ability in comparison with usual plant parts, like seeds, shoots as well as with tissue culture stages including embryogenic and non-embryogenic callus. It was found that nucellar embryos have more regeneration ability as compared to usual plant parts and other tissue culture stages.

Item Type: Article
Uncontrolled Keywords: Apomictic; Fluorescence microscopy; Nucellar embryos; Somatic embryogenesis; Toluidine blue O staining
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 19 Jul 2019 07:15
Last Modified: 19 Jul 2019 07:15
URI: http://eprints.um.edu.my/id/eprint/21658

Actions (login required)

View Item View Item