Recombinase Polymerase Amplification Combined with a Lateral Flow Strip for the Detection of Plasmodium knowlesi

Lai, Meng Yee and Ooi, Choo Huck and Lau, Yee Ling (2018) Recombinase Polymerase Amplification Combined with a Lateral Flow Strip for the Detection of Plasmodium knowlesi. The American Journal of Tropical Medicine and Hygiene, 98 (3). pp. 700-703. ISSN 0002-9637

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Official URL: https://doi.org/10.4269/ajtmh.17-0738

Abstract

The aim of this study was to develop a recombinase polymerase amplification (RPA) combined with a lateral flow (LF) strip method for specific diagnosis of Plasmodium knowlesi. With incubation at 37C, the 18S rRNA gene of P. knowlesi was successfully amplified within 12 minutes. By adding a specifically designed probe to the reaction solution, the amplified RPA product can be visualized on a LF strip. The RPA assay exhibited high sensitivity with limits of detection down to 10 parasites/μL of P. knowlesi. Nonetheless, it was demonstrated that all P. knowlesi (N = 41) and other Plasmodium sp. (N = 25) were positive while negative samples (N = 8) were negative. Therefore, a combination of RPA and LF strip detection is a highly promising approach with the potential to be suitable for use in resource-limited settings.

Item Type: Article
Uncontrolled Keywords: Nucleic Acid Amplification Techniques; Plasmodium knowlesi; Reagent Strips; Recombinases; Sensitivity and Specificity
Subjects: R Medicine
Divisions: Faculty of Medicine
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 27 Jun 2019 03:41
Last Modified: 27 Jun 2019 03:41
URI: http://eprints.um.edu.my/id/eprint/21561

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