Cloning and characterization of short‐chain N ‐acyl homoserine lactone‐producing Enterobacter asburiae strain L1 from lettuce leaves

Lau, Yin Yin and How, Kah Yan and Yin, Wai Fong and Chan, Kok Gan (2018) Cloning and characterization of short‐chain N ‐acyl homoserine lactone‐producing Enterobacter asburiae strain L1 from lettuce leaves. MicrobiologyOpen, 7 (6). e00610. ISSN 2045-8827

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Official URL: https://doi.org/10.1002/mbo3.610

Abstract

In gram-negative bacteria, bacterial communication or quorum sensing (QS) is achieved using common signaling molecules known as N-acyl homoserine lactones (AHL). We have previously reported the genome of AHL-producing bacterium, Enterobacter asburiae strain L1. In silico analysis of the strain L1 genome revealed the presence of a pair of luxI/R genes responsible for AHL-type QS, designated as easIR. In this work, the 639 bp luxI homolog, encoding 212 amino acids, have been cloned and overexpressed in Escherichia coli BL21 (DE3)pLysS. The purified protein (~25 kDa) shares high similarity to several members of the LuxI family among different E asburiae strains. Our findings showed that the heterologously expressed EasI protein has activated violacein production by AHL biosensor Chromobacterium violaceum CV026 as the wild-type E. asburiae. The mass spectrometry analysis showed the production of N-butanoyl homoserine lactone and N–hexanoyl homoserine lactone from induced E. coli harboring the recombinant EasI, suggesting that EasI is a functional AHL synthase. E. asburiae strain L1 was also shown to possess biofilm-forming characteristic activity using crystal violet binding assay. This is the first report on cloning and characterization of the luxI homolog from E. asburiae.

Item Type: Article
Uncontrolled Keywords: AHL synthase; biofilm; Enterobacter asburiae; N-acyl homoserine lactone; protein expression; quorum sensing
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 26 Jun 2019 04:39
Last Modified: 26 Jun 2019 04:39
URI: http://eprints.um.edu.my/id/eprint/21544

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