Complete genome sequence of Rhodothermaceae bacterium RA with cellulolytic and xylanolytic activities

Liew, Kok Jun and Teo, Seng Chong and Shamsir, Mohd Shahir and Sani, Rajesh Kumar and Chong, Chun Shiong and Chan, Kok Gan and Goh, Kian Mau (2018) Complete genome sequence of Rhodothermaceae bacterium RA with cellulolytic and xylanolytic activities. 3 Biotech, 8 (8). p. 376. ISSN 2190-572X

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Official URL: https://doi.org/10.1007/s13205-018-1391-z

Abstract

Rhodothermaceae bacterium RA is a halo-thermophile isolated from a saline hot spring. Previously, the genome of this bacterium was sequenced using a HiSeq 2500 platform culminating in 91 contigs. In this report, we report on the resequencing of its complete genome using a PacBio RSII platform. The genome has a GC content of 68.3%, is 4,653,222 bp in size, and encodes 3711 genes. We are interested in understanding the carbohydrate metabolic pathway, in particular the lignocellulosic biomass degradation pathway. Strain RA harbors 57 glycosyl hydrolase (GH) genes that are affiliated with 30 families. The bacterium consists of cellulose-acting (GH 3, 5, 9, and 44) and hemicellulose-acting enzymes (GH 3, 10, and 43). A crude cell-free extract of the bacterium exhibited endoglucanase, xylanase, β-glucosidase, and β-xylosidase activities. The complete genome information coupled with biochemical assays confirms that strain RA is able to degrade cellulose and xylan. Therefore, strain RA is another excellent member of family Rhodothermaceae as a repository of novel and thermostable cellulolytic and hemicellulolytic enzymes.

Item Type: Article
Uncontrolled Keywords: Cellulase; Halophile; Rhodothermaceae; Rhodothermus; Xylanase
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 17 Jun 2019 04:22
Last Modified: 17 Jun 2019 04:22
URI: http://eprints.um.edu.my/id/eprint/21470

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