Development of Loop-Mediated Isothermal Amplification–Based Lateral Flow Device Method for the Detection of Malaria

Mallepaddi, Prudhvi Chand and Lai, Meng Yee and Podha, Sudhakar and Ooi, Choo Huck and Liew, Jonathan Wee Kent and Polavarapu, Rathnagiri and Lau, Yee Ling (2018) Development of Loop-Mediated Isothermal Amplification–Based Lateral Flow Device Method for the Detection of Malaria. The American Journal of Tropical Medicine and Hygiene, 99 (3). pp. 704-708. ISSN 0002-9637

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Official URL: https://doi.org/10.4269/ajtmh.18-0177

Abstract

The present study aims to develop a method for rapid diagnosis of malaria using loop-mediated isothermal amplification (LAMP) combined with a lateral flow device (LFD). By adding the biotin-labeled and fluorescein amiditelabeled loop primers to the LAMP reaction solution, the end product can be visualized on a LFD. The entire procedure takes approximately 42 minutes tocomplete, LAMP assay exhibited high sensitivity, asthe detection limit was 0.01 pg/μL for all five Plasmodium species. Itwas demonstrated that all Plasmodium knowlesi (N = 90) and Plasmodium vivax(N= 56) were positively amplified by LAMP-LFD assay, whereas healthy donor samples (N = 8) were negative. However, not all mixed infections were positive, and other infected nonmalaria samples were negative. Loop-mediated isothermal amplification-LFD representsarobust approach with potential suitability for useinresource-constrained laboratories. We believe that LAMP-LFD has a potential to be developed as point-of-care diagnostic tool in future.

Item Type: Article
Uncontrolled Keywords: controlled study; lateral flow device; limit of detection; loop mediated isothermal amplification; malaria; mixed infection; nonhuman; Plasmodium knowlesi; Plasmodium vivax; point of care testing; sensitivity analysis
Subjects: R Medicine
Divisions: Faculty of Medicine
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 28 May 2019 03:07
Last Modified: 28 May 2019 03:07
URI: http://eprints.um.edu.my/id/eprint/21352

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