Mallepaddi, Prudhvi Chand and Lai, Meng Yee and Podha, Sudhakar and Ooi, Choo Huck and Liew, Jonathan Wee Kent and Polavarapu, Rathnagiri and Lau, Yee Ling (2018) Development of Loop-Mediated Isothermal Amplification–Based Lateral Flow Device Method for the Detection of Malaria. The American Journal of Tropical Medicine and Hygiene, 99 (3). pp. 704-708. ISSN 0002-9637, DOI https://doi.org/10.4269/ajtmh.18-0177.
Full text not available from this repository.Abstract
The present study aims to develop a method for rapid diagnosis of malaria using loop-mediated isothermal amplification (LAMP) combined with a lateral flow device (LFD). By adding the biotin-labeled and fluorescein amiditelabeled loop primers to the LAMP reaction solution, the end product can be visualized on a LFD. The entire procedure takes approximately 42 minutes tocomplete, LAMP assay exhibited high sensitivity, asthe detection limit was 0.01 pg/μL for all five Plasmodium species. Itwas demonstrated that all Plasmodium knowlesi (N = 90) and Plasmodium vivax(N= 56) were positively amplified by LAMP-LFD assay, whereas healthy donor samples (N = 8) were negative. However, not all mixed infections were positive, and other infected nonmalaria samples were negative. Loop-mediated isothermal amplification-LFD representsarobust approach with potential suitability for useinresource-constrained laboratories. We believe that LAMP-LFD has a potential to be developed as point-of-care diagnostic tool in future.
| Item Type: | Article |
|---|---|
| Funders: | University Malaya PPP Research Grant (PG066-2016A), Department of Biotechnology under SIBRI Project on Malarial Diagnostics (File no: BT/ SBIRI/358/57-B6/2007) |
| Uncontrolled Keywords: | controlled study; lateral flow device; limit of detection; loop mediated isothermal amplification; malaria; mixed infection; nonhuman; Plasmodium knowlesi; Plasmodium vivax; point of care testing; sensitivity analysis |
| Subjects: | R Medicine |
| Divisions: | Faculty of Medicine |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 28 May 2019 03:07 |
| Last Modified: | 28 May 2019 03:07 |
| URI: | http://eprints.um.edu.my/id/eprint/21352 |
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