Successful cleavage of cloned goat embryos using ear fibroblast cell and fetal fibroblast cell as donor karyoplast in interspecies SCNT

Mohd Sharif, Siti Haslinda and Amri, Asdiana and Wan Embong, Wan Khadijah and Abdullah, Ramli (2018) Successful cleavage of cloned goat embryos using ear fibroblast cell and fetal fibroblast cell as donor karyoplast in interspecies SCNT. Indian Journal of Animal Sciences, 88 (9). pp. 1015-1019. ISSN 0367-8318

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Official URL: http://epubs.icar.org.in/ejournal/index.php/IJAnS/...

Abstract

The efficiencies in producing cloned ruminant embryos and subsequent production of offspring are still low. The study was conducted to produce cloned goat and cattle embryos using inter- and intraspecies SCNT techniques. This study involved 3 different types of donor karyoplast using goat and cattle oocyte as a recipient cytoplast to produce cloned goat and cattle embryos. The results showed that fetal fibroblast cell (FFC) in interspecies SCNT gave significantly higher 2-cell (64.40 vs. 38.43%), 4-cell (54.24 vs. 24.60%), 8-cell (36.82 vs. 14.54%) and morula (22.10 vs. 7.90%) cloned goat embryos than ear fibroblast cell (EFC). As for intraspecies SCNT using cumulus cell (CC) as a donor karyoplast to produce cloned cattle and goat embryos, the values for cleavage rates were not significantly different which were; 53.57 vs. 57.17%, 33.17 vs. 46.40%, 22.15 vs. 27.30% and 11.90 vs. 15.59%, respectively for all embryo stages. Our results showed that cloned goat and cattle embryos could be produced using different types of donor karyoplast in intra- and interspecies SCNT. However, for goat-cattle interspecies SCNT, FFC was more efficient to produce cloned goat embryos compared to EFC.

Item Type: Article
Uncontrolled Keywords: Cattle; Donor karyoplast; Goat; Intraspecies SCNT; Interspecies SCNT; Recipient cytoplast
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 14 May 2019 09:00
Last Modified: 14 May 2019 09:00
URI: http://eprints.um.edu.my/id/eprint/21232

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