Testosterone Down-regulates Expression of αVβ3-integrin, E-cadherin and Mucin-1 during Uterine Receptivity Period in Rats

Mohd Mokhtar, Helmy and Giribabu, Nelli and Salleh, Naguib (2018) Testosterone Down-regulates Expression of αVβ3-integrin, E-cadherin and Mucin-1 during Uterine Receptivity Period in Rats. Sains Malaysiana, 47 (10). pp. 2509-2517. ISSN 0126-6039

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Official URL: https://doi.org/10.17576/jsm-2018-4710-28

Abstract

Adequate development of uterine receptivity is crucial for establishment of pregnancy. Expression of uterine receptivity molecules i.e. αvβ3 integrin, E-cadherin and mucin-1 could be affected by testosterone. The objective of this study was to investigate effect of testosterone on expression of these molecules during early pregnancy. 30 ovariectomised female Sprague-Dawley rats were divided into 5 groups that consisted of vehicle control, rats received eight days sex-steroid replacement regime (intended to mimic the hormonal changes in early pregnancy) and three groups of rats given testosterone (1 mg/kg/day) subcutaneously with or without flutamide or finasteride between day 6 and 8 representing the period of uterine receptivity. At the end of the treatment, rats were sacrificed and uteri were removed. Expression and distribution of αvβ3 integrin, E-cadherin and mucin-1 were examined by immunoflourescence and levels of messenger RNA (mRNAs) were evaluated by real-time PCR. Expression of αvβ3 integrin, E-cadherin and mucin-1 in the uteri of rats receiving sex-steroid replacement regime increased significantly as compared to control (p<0.05). In these rats, concomitant administration of testosterone between day 6 and 8 resulted in expression of αvβ3 integrin, E-cadherin and mucin-1 to decrease significantly (p<0.05) as compared to rats receiving sex-steroid replacement regime without testosterone treatment. Moreover, the testosterone effects were not antagonized by either flutamide or finasteride. As a result, reduced expression of uterine receptivity molecules by testosterone might interfere with early pregnancy establishment, therefore could adversely affect the female fertility.

Item Type: Article
Uncontrolled Keywords: E-cadherin; Mucin-1; Testosterone; Uterine receptivity; Αvβ3 integrin
Subjects: R Medicine
Divisions: Faculty of Medicine
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 14 May 2019 08:14
Last Modified: 14 May 2019 08:14
URI: http://eprints.um.edu.my/id/eprint/21225

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