Identification and characterization of neutralization epitopes at VP2 and VP1 of enterovirus A71

Nik Nadia, Nik Mohd Nasir and Tan, Chee Wah and Ong, Kien Chai and Sam, I-Ching and Chan, Yoke Fun (2018) Identification and characterization of neutralization epitopes at VP2 and VP1 of enterovirus A71. Journal of Medical Virology, 90 (6). pp. 1164-1167. ISSN 0146-6615

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Official URL: https://doi.org/10.1002/jmv.25061

Abstract

Enterovirus A71 (EV-A71) neutralization escape mutants were generated with monoclonal antibody MAB979 (Millipore). The VP2-T141I and VP1-D14N substitutions were identified. Using reverse genetics, infectious clones with these substitutions were constructed and tested by neutralization assay with immune sera from mice and humans. The N-terminus VP1-14 is more important than EF loop VP2-141 in acute human infection, mainly because it recognised IgM present in acute infection. The N-terminus VP1 could be a useful target for diagnostics and therapeutic antibodies in acute infection.

Item Type: Article
Uncontrolled Keywords: capsid; enterovirus; enterovirus A71; geneticshand, foot, and mouth disease; immune responses; mutation; neutralization
Subjects: R Medicine
Divisions: Faculty of Medicine
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 07 May 2019 02:20
Last Modified: 07 May 2019 02:20
URI: http://eprints.um.edu.my/id/eprint/21125

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