Antioxidative and cytotoxic activities of crude and isolated compounds of p. Lateriflora (bl.) king

Obaid, Ahmed Kareem and Abdul Azziz, Saripah Salbiah Syed and Bakri, Yuhanis Mhd and Nafiah, Mohd Azlan and Awang, Khalijah and Aowda, Saadon Abdulla and Hassan, Norazian Mohd and Hashim, Yumi Zuhanis Has Yun and Abdullah, Fauziah Hanum (2018) Antioxidative and cytotoxic activities of crude and isolated compounds of p. Lateriflora (bl.) king. Journal of Pharmaceutical Sciences and Research, 10 (11). pp. 2718-2721. ISSN 0975-1459

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Abstract

This study aimed to isolate compounds from Polyalthia lateriflora (Bl.) King stem bark, and to test the crude extracts and the isolated compounds for their antioxidant and cytotoxic activities. Three different solvents were used to prepare the crude extracts; hexane, dichloromethane (DCM) and methanol. Phytochemical investigation of the crude extracts has led to the isolation of four oxoaporphine alkaloids (O-methylmoschatoline, atherospermidine, lysicamine, and liriodenine) and two steroids (stigmasterol and lupeol). The chemical structure of the compounds was elucidated using1 H-NMR,13 C-NMR, 2D-NMR, and mass spectral analysis. All the crude extracts and isolated compounds were tested for their in vitro DPPH radicals scavenging activity and cytotoxicity against MCF-7 breast cancer cells. Methanol extract showed the strongest antioxidant activity with percentage inhibition at 500µg/mL of 89.52±1.81 (IC50, 128.22± 6.73 µg/ml) in comparison with DCM (32.45±8.56), atherospermidine (20.17±9.74) and liriodenine (10.73±0.45), while other extracts were inactive. All the isolated compounds showed cytotoxic against MCF-7 cells, at IC50 values in the range of 42-89 µg/ml. Based on thorough literature review, all compounds identified in this study were isolated for the first time from this plant. In particular, lupeol is reported for the first time in the genus Polyalthia and possessed the most potent cytotoxic effect compared to other isolated compounds.

Item Type: Article
Uncontrolled Keywords: Antioxidant; Cytotoxicity; DPPH scavenger; MCF-7 cells; Polyalthia lateriflora (Bl.) King
Subjects: Q Science > Q Science (General)
Q Science > QD Chemistry
Divisions: Faculty of Science > Dept of Chemistry
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 26 Apr 2019 08:44
Last Modified: 26 Apr 2019 08:44
URI: http://eprints.um.edu.my/id/eprint/21090

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