Immobilization of cross-linked tannase enzyme on multiwalled carbon nanotubes and its catalytic behavior

Ong, Chong Boon and Annuar, Mohamad Suffian Mohamad (2018) Immobilization of cross-linked tannase enzyme on multiwalled carbon nanotubes and its catalytic behavior. Preparative Biochemistry and Biotechnology, 48 (2). pp. 181-187. ISSN 1082-6068

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Official URL: https://doi.org/10.1080/10826068.2018.1425707

Abstract

Immobilization of cross-linked tannase on pristine multiwalled carbon nanotubes (MWCNT) was successfully performed. Cross-linking of tannase molecules was made through glutaraldehyde. The immobilized tannase exhibited significantly improved pH, thermal, and recycling stability. The optimal pH for both free and immobilized tannase was observed at pH 5.0 with optimal operating temperature at 30°C. Moreover, immobilized enzyme retained greater biocatalytic activities upon 10 repeated uses compared to free enzyme in solution. Immobilization of tannase was accomplished by strong hydrophobic interaction most likely between hydrophobic amino acid moieties of the glutaraldehyde-cross-linked tannase to the MWCNT.

Item Type: Article
Additional Information: http://orcid.org/0000-0003-4564-6043
Uncontrolled Keywords: Glutaraldehyde; immobilization; multiwalled carbon nanotubes; tannase
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 25 Apr 2019 03:16
Last Modified: 25 Apr 2019 03:16
URI: http://eprints.um.edu.my/id/eprint/21073

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