Production of N-acyl homoserine lactones by Chromobacterium haemolyticum KM2 isolated from the river water in Malaysia

Priya, Kumutha and Sulaiman, Joanita and How, Kah Yan and Yin, Wai Fong and Chan, Kok Gan (2018) Production of N-acyl homoserine lactones by Chromobacterium haemolyticum KM2 isolated from the river water in Malaysia. Archives of Microbiology, 200 (7). pp. 1135-1142. ISSN 0302-8933

Full text not available from this repository.
Official URL: https://doi.org/10.1007/s00203-018-1526-y

Abstract

Quorum sensing (QS) is a term used to describe cell-to-cell communication that enables bacteria to orchestrate group behaviours according to density of bacterial cells. In Gram-negative bacteria, this signalling system is widely known to regulate a variety of different phenotypes such as antibiotic production and biofilm formation. In this study, we report the production of N-acyl homoserine lactones produced by Chromobacterium haemolyticum strain KM2, a bacterium isolated from a river water of a reserved tropical national park. Preliminary screening of QS activity using biosensor reporter assays indicated that C. haemolyticum strain KM2 produces both short- and long-chain AHLs. Analysis with high-resolution liquid chromatography–mass spectrometry (LC–MS/MS) analysis revealed the production of three AHLs by strain KM2: N-octanoyl-l-homoserine lactone (C8-HSL), N-dodecanoyl-l-homoserine lactone (C12-HSL), and N-3-oxo-dodecanoyl-l-homoserine lactone (OC12-HSL). This bacterial isolate also exhibited strong β-haemolytic activity. To the best of our knowledge, this is the first documentation of QS activity and multiple AHLs production by C. haemolyticum strain KM2.

Item Type: Article
Uncontrolled Keywords: Biosensor; Chromobacterium haemolyticum; N-acyl homoserine lactone; Quorum sensing; Triple quadrupole liquid chromatography–mass spectrometry
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 18 Apr 2019 07:31
Last Modified: 18 Apr 2019 07:31
URI: http://eprints.um.edu.my/id/eprint/21011

Actions (login required)

View Item View Item