Priya, Kumutha and Sulaiman, Joanita and How, Kah Yan and Yin, Wai Fong and Chan, Kok Gan (2018) Production of N-acyl homoserine lactones by Chromobacterium haemolyticum KM2 isolated from the river water in Malaysia. Archives of Microbiology, 200 (7). pp. 1135-1142. ISSN 0302-8933, DOI https://doi.org/10.1007/s00203-018-1526-y.
Full text not available from this repository.Abstract
Quorum sensing (QS) is a term used to describe cell-to-cell communication that enables bacteria to orchestrate group behaviours according to density of bacterial cells. In Gram-negative bacteria, this signalling system is widely known to regulate a variety of different phenotypes such as antibiotic production and biofilm formation. In this study, we report the production of N-acyl homoserine lactones produced by Chromobacterium haemolyticum strain KM2, a bacterium isolated from a river water of a reserved tropical national park. Preliminary screening of QS activity using biosensor reporter assays indicated that C. haemolyticum strain KM2 produces both short- and long-chain AHLs. Analysis with high-resolution liquid chromatography–mass spectrometry (LC–MS/MS) analysis revealed the production of three AHLs by strain KM2: N-octanoyl-l-homoserine lactone (C8-HSL), N-dodecanoyl-l-homoserine lactone (C12-HSL), and N-3-oxo-dodecanoyl-l-homoserine lactone (OC12-HSL). This bacterial isolate also exhibited strong β-haemolytic activity. To the best of our knowledge, this is the first documentation of QS activity and multiple AHLs production by C. haemolyticum strain KM2.
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Uncontrolled Keywords: | Biosensor; Chromobacterium haemolyticum; N-acyl homoserine lactone; Quorum sensing; Triple quadrupole liquid chromatography–mass spectrometry |
| Subjects: | Q Science > Q Science (General) Q Science > QH Natural history |
| Divisions: | Faculty of Science > Institute of Biological Sciences |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 18 Apr 2019 07:31 |
| Last Modified: | 18 Apr 2019 07:31 |
| URI: | http://eprints.um.edu.my/id/eprint/21011 |
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