High Prevalence of Toxigenic and Nontoxigenic Clostridium difficile Strains in Malaysia

Riley, Thomas V. and Collins, Deirdre A. and Karunakaran, Rina and Kahar, Maria Abdul and Adnan, Ariza and Hassan, Siti Asma and Zainul, Nadiah Hanim and Rustam, F. R. Mohammed and Wahab, Z. Abd and Ramli, Ramliza and Lee, Yeong Yeh and Hassan, Hamimah and Onderdonk, Andrew B. (2018) High Prevalence of Toxigenic and Nontoxigenic Clostridium difficile Strains in Malaysia. Journal of Clinical Microbiology, 56 (6). 00170-18. ISSN 0095-1137

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Official URL: https://doi.org/10.1128/JCM.00170-18


Accumulating evidence shows a high prevalence of Clostridium difficile in Southeast Asia associated with a range of clinical presentations. However, severe infections are rarely reported. We investigated C. difficile infection (CDI) across four hospitals in Kuala Lumpur and Kota Bharu, Malaysia. Enzyme immunoassays for glutamate dehydrogenase (GDH) and toxin A or B were performed on diarrheal stool specimens collected from patients in 2015 and 2016. Specimens were also cultured and isolates of C. difficile characterized by PCR ribotyping and detection of toxin genes. In total, 437 specimens were collected and fecal toxin was detected in 3.0%. A further 16.2% of specimens were GDH positive and toxin negative. After culture, toxigenic strains were isolated from 10.3% and nontoxigenic strains from 12.4% of specimens. The most prevalent PCR ribotypes (RTs) were RT 017 (20.0%) and RT 043 (10.0%). The high prevalence of RT 017 and nontoxigenic strains in Malaysia and in neighboring Thailand and Indonesia suggests that they localize to the region of Southeast Asia, with an implication that they may mediate the burden of CDI in the region.

Item Type: Article
Uncontrolled Keywords: Clostridium difficile; Malaysia; epidemiology; prevalence
Subjects: R Medicine
Divisions: Faculty of Medicine
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 10 Apr 2019 07:03
Last Modified: 10 Apr 2019 07:03
URI: http://eprints.um.edu.my/id/eprint/20884

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