Salama, Suzy Munir and Ibrahim, Ibrahim Abdel Aziz and Shahzad, Naiyer and Al-Ghamdi, Saeed and Ayoub, Nahla and AlRashdi, Ahmed Salim and Abdulla, Mahmood Ameen and Salehen, Nur'ain and Bilgen, Mehmet (2018) Hepatoprotectivity of Panduratin A against liver damage: In vivo demonstration with a rat model of cirrhosis induced by thioacetamide. APMIS, 126 (9). pp. 710-721. ISSN 0903-4641, DOI https://doi.org/10.1111/apm.12878.
Full text not available from this repository.Abstract
This experiment evaluated Panduratin A (PA), a chalcone isolated from Boesenbergia rotunda rhizomes, for its hepatoprotectivity. Rats were subjected to liver damage induced by intra-peritoneal injection of thioacetamide (TAA). PA was tested first for its acute toxicity and then administered by oral gavage at doses 5, 10, and 50 mg/kg to rats. At the end of the 8th week, livers from all rats were excised and evaluated ex vivo. Measurements included alkaline phosphatase (AP), alanine transaminase (ALT), aspartate transaminase (AST) and gamma-glutamyl transferase (GGT), serum platelet-derived growth factor (PDGF) and transforming growth factor (TGF-β1), and hepatic metalloproteinase enzyme (MMP-2) and its inhibitor extracellular matrix protein (TIMP-1). Oxidative stress was measured by liver malondialdehyde (MDA) and nitrotyrosine levels, urinary 8-hydroxy 2- deoxyguanosine (8-OH-dG), and hepatic antioxidant enzyme activities. The immunohistochemistry of TGF-β1 was additionally performed. PA revealed safe dose of 250 mg/kg on experimental rats and positive effect on the liver. The results suggested reduced hepatic stellate cells (HSCs) activity as verified from the attenuation of serum PDGF and TGF-β1, hepatic MMP-2 and TIMP-1, and oxidative stress. The extensive data altogether conclude that PA treatment could protect the liver from the progression of cirrhosis through a possible mechanism inhibiting HSCs activity.
| Item Type: | Article |
|---|---|
| Funders: | University of Malaya: (UMRG RPO43A, B-15HTM and RPO21A-14AFR) |
| Uncontrolled Keywords: | Boesenbergia rotunda; cirrhosis; Hepatoprotectivity; Panduratin A; thioacetamide |
| Subjects: | R Medicine |
| Divisions: | Faculty of Medicine |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 03 Apr 2019 09:06 |
| Last Modified: | 03 Apr 2019 09:06 |
| URI: | http://eprints.um.edu.my/id/eprint/20805 |
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