Rapid pulsed field gel electrophoresis method for Streptococcus spp.

Thong, K.L.; Mohd, N.M.D.; Goh, Y.L.; Ngeow, Y.F.; Parasakthi, N. (2001) Rapid pulsed field gel electrophoresis method for Streptococcus spp. Asia Pacific Journal of Molecular Biology & Biotechnology, 9 (2). pp. 143-146.

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Abstract

Pulsed field gel electrophoresis (PFGE) despite being a highly reproducible and discriminatory method for molecular subtyping of many bacterial pathogens has been criticised for being time consuming. We have developed a modified, rapid PFGE method for Gram positive bacteria, in particular, Streptococccus spp. Our method can be completed in only 3 days' time as compared to the standard procedure which required Lip to 6 days. We successfully applied this method on clinical isolates of Streptococcus pneumoniae (50) and Group B Streptococcus (50) obtained from the University of Malaya Medical Center with comparable results. The method for DNA preparation was reproducible when repeated analysis was carried out. The rapid method has the ability to process a large number of isolates in less time than the standard method. This will definitely enhance the rapid and accurate analysis of outbreaks of nosocomial or community acquired Streptococcal infections.

Item Type: Article
Creators:
  1. Thong, K.L.(Institute of Biological Sciences, Faculty of Science Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
  2. Mohd, N.M.D.
  3. Goh, Y.L.
  4. Ngeow, Y.F.
  5. Parasakthi, N.
Journal or Publication Title: Asia Pacific Journal of Molecular Biology & Biotechnology
Additional Information: University of Malaya. Faculty of Science. Institute of Biological Sciences University of Malaya. and Faculty of Medicine. Dept. of Medical Microbiology
Uncontrolled Keywords: Rapid Protocol; PFGE
Subjects: R Medicine
Divisions: Faculty of Science
Depositing User: Mr. Faizal Hamzah
Date Deposited: 05 Sep 2011 12:21
Last Modified: 20 Oct 2014 14:21
URI: http://eprints.um.edu.my/id/eprint/2078

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