Saw, Wen Shang and Ujihara, Masaki and Chong, Wu Yi and Voon, Siew Hui and Imae, Toyoko and Kiew, Lik Voon and Lee, Hong Boon and Sim, Kae Shin and Chung, Lip Yong (2018) Size-dependent effect of cystine/citric acid-capped confeito-like gold nanoparticles on cellular uptake and photothermal cancer therapy. Colloids and Surfaces B: Biointerfaces, 161. pp. 365-374. ISSN 0927-7765, DOI https://doi.org/10.1016/j.colsurfb.2017.10.064.
Full text not available from this repository.Abstract
Physiochemical changes, including size, are known to affect gold nanoparticle cellular internalization and treatment efficacy. Here, we report the effect of four sizes of cystine/citric acid-coated confeito-like gold nanoparticles (confeito-AuNPs) (30, 60, 80 and 100 nm) on cellular uptake, intracellular localization and photothermal anticancer treatment efficiency in MDA-MB231 breast cancer cells. Cellular uptake is size dependent with the smallest size of confeito-AuNPs (30 nm) having the highest cellular internalization via clathrin- and caveolae-mediated endocytosis. However, the other three sizes (60, 80 and 100 nm) utilize clathrin-mediated endocytosis for cellular uptake. The intracellular localization of confeito-AuNPs is related to their endocytosis mechanism, where all sizes of confeito-AuNPs were localized highly in the lysosome and mitochondria, while confeito-AuNPs (30 nm) gave the highest localization in the endoplasmic reticulum. Similarly, a size-dependent trend was also observed in in vitro photothermal treatment experiments, with the smallest confeito-AuNPs (30 nm) giving the highest cell killing rate, whereas the largest size of confeito-AuNPs (100 nm) displayed the lowest photothermal efficacy. Its desirable physicochemical characteristics, biocompatible nature and better photothermal efficacy will form the basis for further development of multifunctional confeito-AuNP-based nanotherapeutic applications.
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Uncontrolled Keywords: | Gold nanoparticles; Cystine; Size; Endocytosis; Intracellular localization; Photothermal cytotoxicity |
| Subjects: | Q Science > Q Science (General) Q Science > QC Physics Q Science > QH Natural history R Medicine |
| Divisions: | Faculty of Medicine Faculty of Science > Department of Physics Faculty of Science > Institute of Biological Sciences |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 19 Mar 2019 07:02 |
| Last Modified: | 19 Mar 2019 07:02 |
| URI: | http://eprints.um.edu.my/id/eprint/20760 |
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