Catabolic activity and biofilm formation of foodborne Listeria monocytogenes strains

Sharar, Nowshin Shyara and Chai, Lay Ching and Thong, Kwai Lin (2018) Catabolic activity and biofilm formation of foodborne Listeria monocytogenes strains. Journal of Consumer Protection and Food Safety, 13 (3). pp. 289-298. ISSN 1661-5751

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Official URL: https://doi.org/10.1007/s00003-018-1169-6

Abstract

Listeria monocytogenes is a major foodborne pathogen causing increased morbidity worldwide. It forms resistant biofilm structures in food processing facilities after sanitization, consequently creating a public health concern. Many studies on the metabolism and transmission of L. monocytogenes has provided insights into its intracellular infection process, however there is limited understanding on the substrate utilization of the bacteria. Therefore, the main objective of this study was to investigate the carbon and nitrogen substrate catabolism and the biofilm forming potential of 3 Malaysian L. monocytogenes strains (LM41, LM92 and LM115) previously isolated from ready-to-eat foods. Biolog Phenotype Microarray (PM) system was used to study the catabolic activity of the foodborne strains in 190 carbon and 380 nitrogen sources. PM analysis showed that the carbon and nitrogen catabolic activity of L. monocytogenes strains were considerably limited and these strains utilised Tween 40 and Tween 80, which are commonly used for the sanitation in food and meat processing industries. Furthermore, all 3 strains showed strong biofilm forming potential in nutrient-rich and nutrient-limited media, irrespective of the serogroups. The data generated could be utilised to develop alternative measure to inhibit biofilm formation in L. monocytogenes in the food processing environment.

Item Type: Article
Uncontrolled Keywords: Biofilm; Foodborne pathogen; Listeria monocytogenes; Phenotype microarray; Ready-to-eat food
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 18 Mar 2019 03:15
Last Modified: 18 Mar 2019 03:15
URI: http://eprints.um.edu.my/id/eprint/20707

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