Mode of Action: Synergistic Interaction of Peppermint (Mentha x piperita L. Carl) Essential Oil and Meropenem Against Plasmid-Mediated Resistant E. coli

Yang, Shun Kai and Yap, Polly Soo Xi and Krishnan, Thiba and Yusoff, Khatijah and Chan, Kok Gan and Yap, Wai Sum and Lai, Kok Song and Lim, Swee Hua Erin (2018) Mode of Action: Synergistic Interaction of Peppermint (Mentha x piperita L. Carl) Essential Oil and Meropenem Against Plasmid-Mediated Resistant E. coli. Records of Natural Products, 12 (6). pp. 582-594. ISSN 1307-6167

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Official URL: https://doi.org/10.25135/rnp.59.17.12.078

Abstract

This study investigated the bactericidal mechanism of peppermint essential oil (PEO) when used singly and in combination with meropenem against multidrug resistant Escherichia coli. Chemical compositions of PEO were identified via GC-MS, followed by time-kill analysis which was performed to evaluate the antibacterial activities of PEO and meropenem. Furthermore, outer membrane permeability test, zeta potential measurement and scanning electron microscopy were performed to evaluate the ability of PEO in bacterial membrane disruption. Next, anti-quorum sensing assay was performed to assess the ability of PEO in quorum sensing inhibition. A complete killing activity was observed within five minutes of treatment with PEO and meropenem at sub-lethal concentrations. In addition, the outer membrane permeability test and zeta potential measurement performed indicated increase in the membrane permeability and membrane disruption which can be observed in the scanning electron micrograph. Furthermore, significant decrease in the light production of E. coli pSB1075 treated by PEO indicates the presence of quorum sensing inhibitors within PEO. The findings suggest that PEO has the ability to disrupt the bacterial outer membrane which increases membrane permeability, in addition to the possible inhibition of bacterial quorum sensing ability in multidrug resistant E. coli, aiding in the reversal of antibiotic resistance.

Item Type: Article
Additional Information: Thiba Krishnan. Ph.D. Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, 50603, Malaysia
Uncontrolled Keywords: Escherichia coli; essential oil; membrane permeability; Mentha x piperita L. Carl; quorum sensing; synergism
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 14 Feb 2019 08:14
Last Modified: 14 Feb 2019 08:14
URI: http://eprints.um.edu.my/id/eprint/20321

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