Yap, Nan Jiun and Vythilingam, Indra and Hoh, Boon Peng and Goh, Xiang Ting and Muslim, Azdayanti and Ngui, Romano and Rajoo, Yamuna and Choy, Seow Huey and William, Timothy and Yeo, Tsin Wen and Lim, Yvonne Ai Lian (2018) Genetic polymorphism and natural selection in the C-terminal 42 kDa region of merozoite surface protein-1 (MSP-1) among Plasmodium knowlesi samples from Malaysia. Parasites & Vectors, 11 (1). p. 3234. ISSN 1756-3305, DOI https://doi.org/10.1186/s13071-018-3234-5.
Full text not available from this repository.Abstract
Background: The merozoite surface protein-1 (MSP-1) gene encodes for a leading malaria vaccine candidate antigen. However, its extensive polymorphic nature represents a major obstacle to the development of a protective vaccine. Previously, a pilot study was carried out to explore the sequence variation of the C-terminal 42 kDa fragment within P. knowlesi MSP-1 gene (PkMSP-142) based on 12 clinical samples; however, further study on an adequate sample size is vital in estimating the genetic diversity of the parasite population. Methods: In the present study, we included a larger sample size of P. knowlesi (83 samples) covering eight states of Malaysia to determine the genetic polymorphism, natural selection and haplotype groups of the gene fragment coding PkMSP-142. The region flanking PkMSP-142 was amplified by PCR and directly sequenced. Genetic diversity, haplotype diversity, population genetic differentiation and natural selection were determined in order to study the polymorphic characteristic of PkMSP-142. Results: A high level of genetic diversity (Hd = 0.970 ± 0.007; cyrillic letter El = 0.01079 ± 0.00033) was observed among the 83 P. knowlesi samples, confirming the extensive genetic polymorphism exhibited among the P. knowlesi population found in Malaysia. A total of 18 distinct haplotypes with 17 amino acid changes were identified, whereby 15 were new haplotypes. High population differentiation values were observed within samples from Peninsular Malaysia and Malaysian Borneo. The 42 kDa fragments of P. knowlesi from Malaysian Borneo were found to be acting on balancing selection whilst purifying selection was suggested to act on isolates from Peninsular Malaysia. The separation of PkMSP-142 haplotypes into two main groups based on geographical separation has further supported the existence of two distinct P. knowlesi lineages. Conclusions: A high level of genetic diversity was observed among PkMSP-142 in Malaysia, whereby most of the polymorphisms were found within the 33 kDa region. Taken together, these data will be useful in order to understand the nature of P. knowlesi population in Malaysia as well as the design and development of a MSP-142 based knowlesi malaria vaccine.
| Item Type: | Article |
|---|---|
| Funders: | University of Malaya Student Grant (PG056-2013A for NJY) and UM/MoHE High Impact Research Grant (H-20001-00-E000061 for YALL), Ministry of Higher Education Malaysia under Fundamental Research Grant Scheme [Grant code: 600-RMI/FRGS 5/3 (136/2014) and FRGS/1/2015/ST03/UCSI/01/1] |
| Uncontrolled Keywords: | Genetic polymorphism; Malaysia; Merozoite surface protein; Natural selection; Plasmodium knowlesi |
| Subjects: | R Medicine |
| Divisions: | Faculty of Medicine |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 14 Feb 2019 04:58 |
| Last Modified: | 26 Aug 2019 04:13 |
| URI: | http://eprints.um.edu.my/id/eprint/20310 |
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