Structural and bone marrow stem cell biocompatibility studies of hydrogel synthesized via chemo-enzymatic route

Latfi, Ahmad Safwan Ahmad and Pramanik, Sumit and Poon, Chi Tat and Gumel, Ahmad Mohammed and Lai, Khin Wee and Annuar, Mohamad Suffian Mohamad and Pingguan-Murphy, Belinda (2019) Structural and bone marrow stem cell biocompatibility studies of hydrogel synthesized via chemo-enzymatic route. Journal of Biomaterials Applications, 33 (6). pp. 854-865. ISSN 0885-3282

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Official URL: https://doi.org/10.1177/0885328218812490

Abstract

Natural biopolymers have many attractive medical applications; however, complications due to fibrosis caused a reduction in diffusion and dispersal of nutrients and waste products. Consequently, severe immunocompatibility problems and poor mechanical and degradation properties in synthetic polymers ensue. Hence, the present study investigates a novel hydrogel material synthesized from caprolactone, ethylene glycol, ethylenediamine, polyethylene glycol, ammonium persulfate, and tetramethylethylenediamine via chemo-enzymatic route. Spectroscopic analyses indicated the formation of polyurea and polyhydroxyurethane as the primary building block of the hydrogel starting material. Biocompatibility studies showed positive observation in biosafety test using direct contact cytotoxicity assay in addition to active cellular growth on the hydrogel scaffold based on fluorescence observation. The synthesized hydrogel also exhibited (self)fluorescence properties under specific wavelength excitation. Hence, synthesized hydrogel could be a potential candidate for medical imaging as well as tissue engineering applications as a tissue expander, coating material, biosensor, and drug delivery system.

Item Type: Article
Uncontrolled Keywords: Polyurea; polyhydroxyurethane; neocell; cytotoxicity; fluorescence; resazurin
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history
R Medicine
Divisions: Faculty of Engineering
Faculty of Science > Institute of Biological Sciences
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 17 Jan 2019 04:25
Last Modified: 27 Feb 2019 00:45
URI: http://eprints.um.edu.my/id/eprint/20041

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