Vasantharaja, Raguraman and Stanley Abraham, L. and Gopinath, Venkatraman and Hariharan, D. and Smita, K.M. (2019) Attenuation of oxidative stress induced mitochondrial dysfunction and cytotoxicity in fibroblast cells by sulfated polysaccharide from Padina gymnospora. International Journal of Biological Macromolecules, 124. pp. 50-59. ISSN 0141-8130, DOI https://doi.org/10.1016/j.ijbiomac.2018.11.104.
Full text not available from this repository.Abstract
In this present study, isolation, characterization and protective effect of sulfated polysaccharide (SP) isolated from the brown algae Padina gymnospora was investigated. SP was isolated and characterized through FT-IR, 1H NMR, TGA, GC–MS and CHN analysis. The molecular weight of SP was found to be 16 kDa. The isolated SP contains 29.4 ± 0.35% of sulfate, 27 ± 0.11% of fucose, 0.05 ± 0.12% of protein, respectively. Furthermore, SP exhibits its excellent radical scavenging effects were evaluated by DPPH, ABTS radical scavenging and reducing power assays. Moreover, pretreatment with SP significantly mitigates H2O2 induced cytotoxicity in L-929 cells in a dose dependent manner. Furthermore, SP pretreatment ameliorates oxidative stress induced apoptosis and DNA damage, alleviates the generation of intracellular reactive oxygen species (ROS) and restores mitochondrial membrane potential (MMP) in L-929 cells through its antioxidant potential. Together, these results suggest that SP can be exploited as a natural antioxidant in the food and pharmaceutical industries.
| Item Type: | Article |
|---|---|
| Funders: | Atomic Energy Regulatory Board: funding support (No. AERB/CSRP/Proj. No. 60/08/2015) |
| Uncontrolled Keywords: | Padina gymnospora; Sulfated polysaccharide; Oxidative stress; Antioxidants; Reactive oxygen species |
| Subjects: | R Medicine |
| Divisions: | Faculty of Medicine |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 14 Jan 2019 04:25 |
| Last Modified: | 14 Jan 2019 04:25 |
| URI: | http://eprints.um.edu.my/id/eprint/19973 |
Actions (login required)
 |
View Item |
