Yu, Shu Pin and Ong, Kien Chai and Perera, David and Wong, Kum Thong (2019) Neuronal transcriptomic responses to Japanese encephalitis virus infection with a special focus on chemokine CXCL11 and pattern recognition receptors RIG-1 and MDA5. Virology, 527. pp. 107-115. ISSN 0042-6822, DOI https://doi.org/10.1016/j.virol.2018.10.015.
Full text not available from this repository.Abstract
Japanese encephalitis virus (JEV) causes central nervous system neuronal injury and inflammation. A clear understanding of neuronal responses to JEV infection remains elusive. Using the Affymetrix array to investigate the transcriptome of infected SK-N-MC cells, 1316 and 2737 dysregulated genes (≥ 2/−2 fold change, P < 0.05) were found at 48 hours post-infection (hpi) and 60 hpi, respectively. The genes were mainly involved in anti-microbial responses, cell signalling, cellular function and maintenance, and cell death and survival. Among the most highly upregulated genes (≥ 10 folds, P < 0.05) were chemokines CCL5, CXCL11, IL8 and CXCL10. The upregulation and expression of CXCL11 were confirmed by qRT-PCR and immunofluorescence. Pathogen recognition receptors retinoic acid-inducible gene-1 (RIG-1) and melanoma differentiation-associated protein 5 (MDA5) were also upregulated. Our results strongly suggest that neuronal cells play a significant role in immunity against JEV. CXCL11, RIG-1 and MDA5 and other cytokines may be important in neuropathogenesis.
| Item Type: | Article |
|---|---|
| Funders: | HIR grant, University of Malaya, Malaysia [Project number: UM.C/625/1/HIR/MOHE/MED/06], Postgraduate Research Fund, University of Malaya, Malaysia [Project number: PG285–2016A] |
| Uncontrolled Keywords: | Japanese encephalitis virus; Transcriptome; RNA microarray; Proinflammatory mediators; Neuronal infection; CXCL11; RIG-1; MDA5 |
| Subjects: | R Medicine |
| Divisions: | Faculty of Medicine |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 14 Jan 2019 03:53 |
| Last Modified: | 14 Jan 2019 03:53 |
| URI: | http://eprints.um.edu.my/id/eprint/19968 |
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