Comparative SELDI-TOF-MS profiling of low-molecular-mass proteins from Lignosus rhinocerus (Cooke) Ryvarden grown under stirred and static conditions of liquid fermentation.

Lau, B.F.; Aminudin, N.; Abdullah, N. (2011) Comparative SELDI-TOF-MS profiling of low-molecular-mass proteins from Lignosus rhinocerus (Cooke) Ryvarden grown under stirred and static conditions of liquid fermentation. Journal of Microbiological Methods. ISSN 0167-7012

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Abstract

Mushrooms are considered as important source of biologically active compounds which include low-molecular-mass protein/peptides (LMMP). In this study, we attempted to profile the LMMP from Lignosus rhinocerus, a wild medicinal mushroom, grown by static cultures (SC) and in stirred tank reactor (STR). Crude water extract (CWE) and protein fractions were profiled using H50 ProteinChipĀ® arrays and SELDI-TOF-MS. Three protein peaks of 5.8, 6.9 and 9.1kDa were found to be common to spectra of L. rhinocerus CWE from both culture conditions. Partial protein purification has resulted in detection of more peaks in the spectra of protein fractions. For protein fractions of L. rhinocerus cultured in STR, most peaks were observed in the range of 3-8kDa whereas some peaks with molecular mass up to 14.3kDa were noted in spectra of protein fractions from SC. Our results have demonstrated the optimization of profiling method using SELDI-TOF-MS for fungal LMMP.

Item Type: Article
Creators:
  1. Lau, B.F.(University of Malaya)
  2. Aminudin, N.(University of Malaya)
  3. Abdullah, N.(University of Malaya)
Journal or Publication Title: Journal of Microbiological Methods
Additional Information: Mushroom Research Centre, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia
Uncontrolled Keywords: Mushrooms
Subjects: R Medicine
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Mr. Faizal Hamzah
Date Deposited: 08 Aug 2011 12:49
Last Modified: 16 Dec 2014 12:25
URI: http://eprints.um.edu.my/id/eprint/1957

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