Thermo-kinetics of lipase-catalyzed synthesis of 6-O-glucosyldecanoate.

Gumel, A.M.; Annuar, M.S.; Heidelberg, T.; Chisti, Y. (2011) Thermo-kinetics of lipase-catalyzed synthesis of 6-O-glucosyldecanoate. Bioresource Technology. ISSN 0960-8524

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Abstract

Lipase-catalyzed synthesis of 6-O-glucosyldecanoate from d-glucose and decanoic acid was performed in dimethyl sulfoxide (DMSO), a mixture of DMSO and tert-butanol and tert-butanol alone with a decreasing order of polarity. The highest conversion yield (>65%) of decanoic acid was obtained in the blended solvent of intermediate polarity mainly because it could dissolve relatively large amounts of both the reactants. The reaction obeyed Michaelis-Menten type of kinetics. The affinity of the enzyme towards the limiting substrate (decanoic acid) was not affected by the polarity of the solvent, but increased significantly with temperature. The esterification reaction was endothermic with activation energy in the range of 60-67kJmol(-1). Based on the Gibbs energy values, in the solvent blend of DMSO and tert-butanol the position of the equilibrium was shifted more towards the products compared to the position in pure solvents. Monoester of glucose was the main product of the reaction.

Item Type: Article
Journal or Publication Title: Bioresource Technology
Additional Information: Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia
Uncontrolled Keywords: Thermo-kinetics
Subjects: R Medicine
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Mr. Faizal Hamzah
Date Deposited: 08 Aug 2011 09:12
Last Modified: 18 Nov 2013 10:14
URI: http://eprints.um.edu.my/id/eprint/1949

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