Gumel, A.M.; Annuar, M.S.; Heidelberg, T.; Chisti, Y. (2011) Thermo-kinetics of lipase-catalyzed synthesis of 6-O-glucosyldecanoate. Bioresource Technology. ISSN 0960-8524Full text not available from this repository.
Lipase-catalyzed synthesis of 6-O-glucosyldecanoate from d-glucose and decanoic acid was performed in dimethyl sulfoxide (DMSO), a mixture of DMSO and tert-butanol and tert-butanol alone with a decreasing order of polarity. The highest conversion yield (>65%) of decanoic acid was obtained in the blended solvent of intermediate polarity mainly because it could dissolve relatively large amounts of both the reactants. The reaction obeyed Michaelis-Menten type of kinetics. The affinity of the enzyme towards the limiting substrate (decanoic acid) was not affected by the polarity of the solvent, but increased significantly with temperature. The esterification reaction was endothermic with activation energy in the range of 60-67kJmol(-1). Based on the Gibbs energy values, in the solvent blend of DMSO and tert-butanol the position of the equilibrium was shifted more towards the products compared to the position in pure solvents. Monoester of glucose was the main product of the reaction.
|Journal or Publication Title:||Bioresource Technology|
|Additional Information:||Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia|
|Divisions:||Faculty of Science > Institute of Biological Sciences|
|Depositing User:||Mr. Faizal Hamzah|
|Date Deposited:||08 Aug 2011 09:12|
|Last Modified:||18 Nov 2013 10:14|
Actions (login required)