Intrinsic Fluorescence as a Spectral Probe for Protein Denaturation Studies in the Presence of Honey

Wong, Y.H. and Kadir, H.A. and Tayyab, S. (2015) Intrinsic Fluorescence as a Spectral Probe for Protein Denaturation Studies in the Presence of Honey. Journal of Applied Spectroscopy, 82 (5). pp. 845-848. ISSN 0021-9037

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Official URL: http://dx.doi.org/10.1007/s10812-015-0191-z

Abstract

Honey was found to quench the intrinsic fluorescence of bovine serum albumin (BSA) in a concentration dependent manner, showing complete quenching in the presence of 5% (w/v) honey. Increasing the protein concentration up to 5.0 μM did not lead to the recovery of the protein fluorescence. Urea denaturation of BSA, which otherwise shows a two-step, three-state transition, using intrinsic fluorescence of the protein as the probe failed to produce any result in the presence of 5% (w/v) honey. Thus, intrinsic fluorescence cannot be used as a spectral probe for protein denaturation studies in the presence of honey.

Item Type: Article
Uncontrolled Keywords: Bovine serum albumin; Fluorescence spectroscopy; Fluorimetric interference; Honey; Protein denaturation
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 26 Sep 2018 07:42
Last Modified: 26 Sep 2018 07:42
URI: http://eprints.um.edu.my/id/eprint/19431

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