Wong, Y.H. and Kadir, H.A. and Tayyab, S. (2015) Intrinsic Fluorescence as a Spectral Probe for Protein Denaturation Studies in the Presence of Honey. Journal of Applied Spectroscopy, 82 (5). pp. 845-848. ISSN 0021-9037, DOI https://doi.org/10.1007/s10812-015-0191-z.
Full text not available from this repository.Abstract
Honey was found to quench the intrinsic fluorescence of bovine serum albumin (BSA) in a concentration dependent manner, showing complete quenching in the presence of 5% (w/v) honey. Increasing the protein concentration up to 5.0 μM did not lead to the recovery of the protein fluorescence. Urea denaturation of BSA, which otherwise shows a two-step, three-state transition, using intrinsic fluorescence of the protein as the probe failed to produce any result in the presence of 5% (w/v) honey. Thus, intrinsic fluorescence cannot be used as a spectral probe for protein denaturation studies in the presence of honey.
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Uncontrolled Keywords: | Bovine serum albumin; Fluorescence spectroscopy; Fluorimetric interference; Honey; Protein denaturation |
| Subjects: | Q Science > Q Science (General) Q Science > QH Natural history |
| Divisions: | Faculty of Science > Institute of Biological Sciences |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 26 Sep 2018 07:42 |
| Last Modified: | 26 Sep 2018 07:42 |
| URI: | http://eprints.um.edu.my/id/eprint/19431 |
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